The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components
The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components
复制标题
DOI：
10.1073/pnas.0509333103
复制
发表时间：
2006-02-21
影响因子：
11.1
通讯作者：
Sassone-Corsi, P
中科院分区：
文献类型：
--
作者：
Kotaja, N;Bhattacharyya, SN;Sassone-Corsi, P
类染色体是雄性生殖细胞中的一种核周、胞质云状结构，其功能至今仍不清楚。在这里，我们表明，拟染色体体与体细胞的RNA加工体有关。Dicer和microRNP复合物的组分（包括Ago蛋白和microRNA）高度集中在拟染色体体中。此外，我们表明，切丁酶与生殖细胞特异性拟染色体体成分，RNA解旋酶MVH（小鼠VASA同系物）相互作用。因此，拟染色体似乎作为microRNA途径的细胞内神经中枢。我们的研究结果强调了转录后基因调控和microRNA途径在控制减数分裂后雄性生殖细胞分化中的重要性。
The chromatoid body is a perinuclear, cytoplasmic cloud-like structure in male germ cells whose function has remained elusive. Here we show that the chromatoid body is related to the RNA-processing body of somatic cells. Dicer and components of microRNP complexes (including Ago proteins and microRNAs) are highly concentrated in chromatoid bodies. Furthermore, we show that Dicer interacts with a germ cell-specific chromatoid body component, the RNA helicase MVH (mouse VASA homolog). Thus, chromatoid bodies seem to operate as intracellular nerve centers of the microRNA pathway. Our findings underscore the importance of posttranscriptional gene regulation and of the microRNA pathway in the control of postmeiotic male germ cell differentiation.