SUBCELLULAR-LOCALIZATION OF PHOSPHATIDYLINOSITOL SYNTHASE FROM GERMINATING SOYBEANS
SUBCELLULAR-LOCALIZATION OF PHOSPHATIDYLINOSITOL SYNTHASE FROM GERMINATING SOYBEANS
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DOI：
10.1111/j.1745-4514.1980.tb00653.x
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发表时间：
1980-01-01
影响因子：
4
通讯作者：
DOUGHERTY, M
中科院分区：
文献类型：
--
作者：
CARMAN, GM;DOUGHERTY, M
CDP-1，2-二酰基-sn-甘油：肌肌醇磷脂酰转移酶（EC. 2.7.8.11，磷脂酰肌醇合酶）催化磷脂酰肌醇从头合成的最后一步。用差速离心和蔗糖梯度离心法对萌发大豆中的这种酶进行了亚细胞定位。粗提物的差速离心结果表明，磷脂酰肌醇合成酶活性主要集中在12，000 ×g ~ 100，000 ×g的沉淀组分中。蔗糖梯度离心的粗提物表明，磷脂酰肌醇合酶与内质网。
CDP‐1,2‐diacyl‐sn‐glycerol:myo‐inositol phosphatidyltransferase (EC.2.7.8.11,phosphatidylinositol synthase) catalyzes the final step in thede novosynthesis of phosphatidylinositol. The subcellular localization of this enzyme from germinating soybeans was determined by differential centrifugation and sucrose gradient centrifugation. Differential centrifugation of the crude extract indicated that phosphatidylinositol synthase activity was primarily located in the fraction sedimenting between12,000 ×g and100,000 ×g. Sucrose gradient centrifugation of the crude extract indicated that phosphatidylinositol synthase was associated with the endoplasmic reticulum.