STRUCTURE + ASSEMBLY OF MACROMOLECULAR LIPID COMPLEXES COMPOSED OF GLOBULAR MICELLES
STRUCTURE + ASSEMBLY OF MACROMOLECULAR LIPID COMPLEXES COMPOSED OF GLOBULAR MICELLES
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DOI：
10.1016/s0022-2836(64)80121-2
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发表时间：
1964-01-01
影响因子：
5.6
通讯作者：
GLAUERT, AM
中科院分区：
文献类型：
--
作者：
LUCY, JA;GLAUERT, AM
已通过电子显微镜研究了卵磷脂、胆固醇和皂苷的各种混合物的负染色制剂。层状，管状，六角形和螺旋结构，它已被观察到，许多这些结构可以令人满意地解释只有在小球状脂质胶束。假定存在两种类型的胶束，一种主要含有卵磷脂和胆固醇，另一种主要含有皂苷和胆固醇，可测量的直径分别为约40 A和约35 A。这些胶束似乎在水性分散体中自发聚集以形成观察到的复杂结构，并且认为胶束之间的氢键和静电相互作用是完整组装体稳定性的原因。使用短链合成卵磷脂制备的螺旋结构含有相应减小尺寸的亚单元。卵磷脂和胆固醇的混合物中存在的管状结构在外观和尺寸上类似于细菌鞭毛，而卵清蛋白、胆固醇和皂苷的混合物中的螺旋类似于烟草花叶病毒。一些这些观察的影响进行了讨论，并建议，小球状胶束，以及双分子小叶，可以作为积木在形成含有脂质的生物结构。
Negatively-stained preparations of various mixtures of lecithin, cholesterol and saponin have been investigated by electron microscopy. Lamellar, tubular, hexagonal and helical structures were observed and it has been concluded that many of these structures can be satisfactorily interpreted only in terms of small globular lipid micelles. It is postulated that two types of micelle are present one containing primarily lecithin and cholesterol, and the other containing mainly saponin and cholesterol, with measurable diameters of about 40 A and about 35 A respectively. These micelles appear to aggregate spontaneously in aqueous dispersions to form the observed complex structures, and it is thought that hydrogen bonds and electrostatic interactions between micelles are responsible for the stability of the complete assemblies. Helical structures prepared using a short-chain synthetic lecithin contain sub units of correspondingly reduced dimensions. The tubular structures present in mixtures of lecithin and cholesterol are similar in appearance and dimensions to bacterial flagella, while helices in mixtures of ovolecithin, cholesterol and saponin resemble tobacco mosaic viruses. Some of the implications of these observations are discussed, and it is suggested that small globular micelles, as well as bimolecular leaflets, may function as building blocks in the formation of biological structures containing lipids.