Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women.
Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women.
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DOI：
10.1136/gutjnl-2012-303304
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发表时间：
2013-08
期刊：
影响因子：
24.5
通讯作者：
Delzenne NM
中科院分区：
文献类型：
--
作者：
Dewulf EM;Cani PD;Claus SP;Fuentes S;Puylaert PG;Neyrinck AM;Bindels LB;de Vos WM;Gibson GR;Thissen JP;Delzenne NM
强调肠道微生物群对肥胖女性通过饮食菊粉型果聚糖（ITF益生元）调节宿主代谢的贡献。对用ITF益生元（菊粉/低聚果糖50/50混合物; n=15）或安慰剂（麦芽糖糊精; n =15）治疗3个月（16 g/天）的30名肥胖妇女进行双盲、安慰剂对照、干预研究。 治疗前后分别进行血、粪、尿采样、口服葡萄糖耐量试验、稳态模型评估和阻抗测定。通过系统发育微阵列和16 S rDNA的qPCR分析来分析粪便中的肠道微生物组成。通过1H-NMR光谱分析血浆和尿液代谢特征。用ITF益生元治疗，而不是安慰剂，导致双歧杆菌和普氏粪杆菌的增加;这两种细菌与血清脂多糖水平呈负相关。ITF益生元还减少了嗜酸拟杆菌、普通拟杆菌和丙酸杆菌，这种作用与脂肪量以及血浆乳酸和磷脂酰胆碱水平的轻微降低有关。对于肠道微生物分析或血浆和尿液代谢组学分析，未检测到明确的治疗聚类。然而，ITF益生元导致肠道微生物群的微妙变化，这可能对与肥胖和/或糖尿病有关的几种关键代谢物产生重要影响。ITF益生元选择性地改变了肥胖女性的肠道微生物群组成，导致宿主代谢的适度变化，正如某些细菌物种与代谢内毒素血症或代谢组学特征之间的相关性所表明的那样。
To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.