CHARACTERIZATION OF BOVINE MHC CLASS-II POLYMORPHISM USING 3 TYPING METHODS - SEROLOGY, RFLP AND IEF
CHARACTERIZATION OF BOVINE MHC CLASS-II POLYMORPHISM USING 3 TYPING METHODS - SEROLOGY, RFLP AND IEF
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DOI：
10.1111/j.1744-313x.1992.tb00068.x
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发表时间：
1992-10-01
期刊：
影响因子：
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通讯作者：
HENSEN, EJ
中科院分区：
文献类型：
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作者：
DAVIES, CJ;ANDERSSON, L;HENSEN, EJ
牛主要组织相容性复合体（MHC）II类基因多态性的研究方法多种多样，各有优缺点。牛淋巴细胞抗原（BoLA）单倍型的一个更完整的表征，可以通过结合这些方法。在这项研究中，BoLA II类多态性的特点是使用三种分型方法：血清学，限制性片段长度多态性（RFLP）和等电聚焦（IEF）。选择26头荷斯坦-弗里斯兰牛和15头安格斯牛进行研究，这些牛携带一系列血清学定义的BoLA单倍型。该组包括12个BoLA复合物纯合子。三种II类分型方法识别与DQ-DR II类亚区中相同或非常紧密连锁的基因相关的多态性。共确定了25种BoLA-A基因座（I类）-DQ-DR亚区（II类）单倍型。三个血清学II类特异性，Dx 1，Dx 3和Dx 4，与一个以上的RFLP定义的DQ-DR单倍型。其他4个II类特异性表现为私人特异性。在荷斯坦牛和安格斯牛中均发现一种BoLA单倍型。这里定义的另外两个BoLA单倍型先前在其他品种中描述过。这表明这些单倍型存在很强的连锁不平衡。
Various methods, with different strengths and weaknesses, are currently used to define polymorphism of the bovine major histocompatibility complex (MHC) class II genes. A more complete characterization of bovine lymphocyte antigen (BoLA) haplotypes can be achieved by combining several of these methods. In this study BoLA class II polymorphism was characterized using three typing methods: serology, restriction fragment length polymorphism (RFLP), and isoelectric focusing (IEF). Twenty six Holstein-Friesian and 15 Angus cattle that carried an array of serologically defined BoLA haplotypes were selected for the study. The panel included 12 BoLA complex homozygotes. The three class II typing methods recognized polymorphism associated with the same or very tightly linked genes in the DQ-DR class II subregion. In total 25 BoLA-A locus (class I) - DQ-DR subregion (class II) haplotypes were defined. Three of the serological class II specificities, Dx1, Dx3, and Dx4, were associated with more than one RFLP defined DQ-DR haplotype. The other 4 class II specificities behaved as private specificities. One BoLA haplotype was found in both Holstein and Angus cattle. Two other BoLA haplotypes defined here have previously been described in other breeds. This suggests that these haplotypes exist in strong linkage disequilibrium.