Characterisation of bovine strains of Pasteurella multocida and comparison with isolates of avian, ovine and porcine origin
Characterisation of bovine strains of Pasteurella multocida and comparison with isolates of avian, ovine and porcine origin
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DOI：
10.1016/j.vetmic.2003.11.013
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发表时间：
2004-04-05
影响因子：
3.3
通讯作者：
Reilly, S
中科院分区：
文献类型：
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作者：
Davies, RL;MacCorquodale, R;Reilly, S
153牛多杀性巴氏杆菌菌株回收主要从肺炎和乳腺炎的情况下，在英格兰和威尔士超过11年的时间内，其特征在于荚膜PCR分型，比较外膜蛋白（OMP）的配置文件，和多位点序列分析。所有菌株均为A型荚膜，只有一株F型荚膜分离株除外。13个不同的外膜蛋白谱（外膜蛋白类型），主要是基于热修饰（OmpA）和孔蛋白（OmpH）的分子量异质性。然而，85%的菌株仅由五种OMP型代表，39%的菌株为单一OMP型。多位点序列分析显示，牛多杀性巴氏杆菌分离株之间的遗传多样性程度有限;相同OMP型的菌株具有相同的遗传背景，代表不同的克隆。OMP变异分析比多位点序列分析更具鉴别力，因为不同OMP类型的菌株具有相同或相似的遗传背景。少数克隆与大多数牛肺炎病例的关联表明，与不太频繁恢复的克隆相比，这些克隆具有更高的致病能力。OmpA和OmpH在相同或相似遗传背景的菌株中的分子量异质性表明，这些蛋白质在宿主内受到多样化选择，并且可能在宿主-病原体相互作用中起重要作用。绵羊和猪的菌株表明，在这些物种中的每一个中，高比例的呼吸道感染是由多杀性巴斯德菌的不同菌株引起的。然而，在一个以上的宿主物种中存在少量密切相关的菌株表明，不同宿主物种之间的细菌传播也是多杀性巴斯德菌种群生物学的一个因素。(C)2004 Elsevier B. V.保留所有权利。
One hundred and fifty-three bovine Pasteurella multocida strains recovered primarily from cases of pneumonia and mastitis in England and Wales over an 11-year period were characterised by capsular PCR typing, comparison of outer membrane protein (OMP) profiles, and multilocus sequence analysis. All of the strains were of capsular type A with the exception of a single capsular type F isolate. Thirteen distinct OMP profiles (OMP-types) were identified based mainly on molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. However, 85% of the isolates were represented by just five OMP-types and 39% of the strains were of a single OMP-type. Multilocus sequence analysis revealed a limited degree of genetic diversity among bovine P. multocida isolates; strains of the same OMP-type have identical genetic backgrounds and represent distinct clones. Analysis of OMP variation was more discriminating than multilocus sequence analysis because strains of different OMP-types had the same, or similar, genetic backgrounds. The association of a small number of clones with the majority of cases of bovine pneumonia suggests that these clones have an increased capacity to cause disease compared to less frequently recovered clones. Molecular mass heterogeneity of OmpA and OmpH, in strains of the same or similar genetic background, suggests that these proteins are subject to diversifying selection within the host and might play important roles in host-pathogen interactions, Comparison of the OMP profiles of bovine isolates with those of avian, ovine and porcine strains showed that a high proportion of the respiratory tract infections in each of these species are caused by different strains of P multocida. However, the presence of small numbers of closely related strains in more than one host species suggests that transmission of bacteria between different host species is also a factor in the population biology of P multocida. (C) 2004 Elsevier B.V. All rights reserved.