Reduced DNA repair in mouse satellite DNA after treatment with methylmethanesulfonate, and N-methyl-N-nitrosourea.
Reduced DNA repair in mouse satellite DNA after treatment with methylmethanesulfonate, and N-methyl-N-nitrosourea.
复制标题
用甲基甲磺酸盐和 N-甲基-N-亚硝基脲处理后，小鼠卫星 DNA 的 DNA 修复减少。
作者：
W. Bodell;M. Banerjee
我们已经测量了小鼠卫星和主带DNA的DNA修复，通过Ag+-Cs2 SO 4离心反应与烷化剂，甲基甲磺酸酯，和N-甲基-N-亚硝基脲治疗解决。我们发现，有一个统计学上显着较低的掺入到卫星DNA相比，在不同时期的烷基化剂处理后的主带。这表明卫星DNA中的修复活性降低。我们已经测量了14 C-甲基甲磺酸盐与卫星DNA和主带DNA的结合程度，没有观察到结合的差异，表明卫星DNA的修复活性降低不是由于烷化剂结合的差异。我们认为，3 H-Tdr掺入卫星DNA的减少可能是由于其在浓缩的染色质组分的位置。
We have measured DNA repair in mouse satellite and main band DNA as resolved by Ag+-Cs2SO4 centrifugation in response to treatment with the alkylating agents, methyl methanesulfonate, and N-methyl-N-nitrosourea. We find that there is a statistically significant lower incorporation of 3H-Tdr into the satellite DNA as compared to the main band at varying periods after treatment with the alkylating agents. This suggests a reduced repair activity in the satellite DNA. We have measured the extent of binding of 14C-methyl methanesulfonate to the satellite, and main band DNA, and no difference in binding was observed, indicating that the reduced repair activity of satellite DNA is not due to a difference in binding of alkylating agents. We believe that the reduced incorporation of 3H-Tdr into satellite DNA may be due to its location in the condensed chromatin fraction.