Assembly and disassembly of the peripheral architecture of the plant cell nucleus during mitosis
Assembly and disassembly of the peripheral architecture of the plant cell nucleus during mitosis
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DOI：
10.1007/s004250050666
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发表时间：
1999-11-01
期刊：
影响因子：
4.3
通讯作者：
Fujino, K
中科院分区：
文献类型：
--
作者：
Masuda, K;Haruyama, S;Fujino, K
高等植物的细胞核的结构包括类似于脊椎动物的核纤层的结构。在胡萝卜（Daucus carota L.）和芹菜（Apium graveolens L.）使用识别核基质蛋白NMCP 1的抗体通过免疫荧光显微镜观察细胞。这种蛋白质已被证明只局限于细胞核的周边（K。增田（Masuda）等人，1997，实验细胞研究（Exp Cell Res）232：173-187）。免疫荧光在有丝分裂中期的整个细胞中被识别，尽管它主要分布在有丝分裂纺锤体区。在后期或末期，免疫荧光定位于每一组子染色体周围。新形成的子核中的免疫荧光仅限于核的周边。这一行为与脊椎动物的核纤层非常相似，表明植物中位于核膜与染色体之间的结构与核膜的解体和重组是平行的。
The architecture of the nuclei of higher plants includes a structure similar to the nuclear lamina of vertebrates. Changes in this structure were monitored during mitosis in carrot (Daucus carota L.) and celery (Apium graveolens L.) cells by immunofluorescence microscopy using an antibody that recognized the nuclear-matrix protein NMCP1. This protein has been shown to be localized exclusively at the periphery of the nucleus (K. Masuda et al. 1997, Exp Cell Res 232: 173-187). Immunofluorescence was recognized throughout cells in mitotic metaphase, although it was distributed predominantly in the mitotic spindle zone. At late anaphase or telophase, the immunofluorescence was localized around each set of daughter chromosomes. Immunofluorescence in newly formed daughter nuclei was restricted to the periphery of nuclei. This behavior was very similar to that of the nuclear lamina of vertebrates, suggesting that the structure located between the nuclear envelope and the chromosomes in plants disassembles and assembles in parallel with the disintegration and re- formation of the nuclear envelope.