Up-regulation of MCM3 Relates to Neuronal Apoptosis After Traumatic Brain Injury in Adult Rats
Up-regulation of MCM3 Relates to Neuronal Apoptosis After Traumatic Brain Injury in Adult Rats
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MCM3 的上调与成年大鼠脑外伤后神经元凋亡有关
DOI：
10.1007/s10571-016-0404-x
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发表时间：
2017-05-01
影响因子：
4
通讯作者：
Gao, Yilu
中科院分区：
文献类型：
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作者：
Ji, Wei;Liu, Hanzhang;Gao, Yilu
微小染色体维持复合体成分3是微小染色体维持蛋白的一种，作为复制前复合体的一部分，在真核生物中启动DNA复制。微小染色体维持复合体3(MCM3)主要参与细胞增殖和肿瘤发生。此外，MCM3可能在神经元凋亡中起重要作用。然而，目前对MCM3在中枢神经系统中的作用还知之甚少。在这项研究中，我们在成年大鼠上建立了创伤性脑损伤(TBI)模型。Western印迹和免疫组织化学染色显示MCM3在损伤周围大脑皮层表达上调。活化的caspase-3和Bax、Bcl2的表达模式与MCM3的表达模式平行。免疫荧光染色和末端脱氧核苷酸转移酶介导的生物素化dUTP缺口末端标记表明MCM3参与了神经元的凋亡。综上所述，我们的研究结果表明，MCM3可能在脑外伤后神经细胞的凋亡中起重要作用。对这些见解的进一步了解可以作为扩大脑外伤治疗范围的基础。
Minichromosome maintenance complex component 3, one of the minichromosome maintenance proteins, functions as a part of pre-replication complex to initiate DNA replication in eukaryotes. Minichromosome maintenance complex component 3 (MCM3) was mainly implied in cell proliferation and tumorigenesis. In addition, MCM3 might play an important role in neuronal apoptosis. However, the functions of MCM3 in central nervous system are still with limited acquaintance. In this study, we performed a traumatic brain injury (TBI) model in adult rats. Western blot and immunohistochemistry staining showed up-regulation of MCM3 in the peritrauma brain cortex. The expression patterns of active caspase-3 and Bax, Bcl-2 were parallel with that of MCM3. Immunofluorescent staining and terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling suggested that MCM3 was involved in neuronal apoptosis. In conclusion, our data indicated that MCM3 might play an important role in neuronal apoptosis following TBI. Further understanding of these insights could serve as the basis for broadening the therapeutic scope against TBI.