Redefinition of the map position and validation of a major quantitative trait locus for fire blight resistance of the pear cultivar 'Harrow Sweet' (Pyrus communis L.)
Redefinition of the map position and validation of a major quantitative trait locus for fire blight resistance of the pear cultivar 'Harrow Sweet' (Pyrus communis L.)
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DOI：
10.1111/j.1439-0523.2012.02000.x
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发表时间：
2012-10-01
期刊：
影响因子：
2
通讯作者：
Patocchi, Andrea
中科院分区：
文献类型：
--
作者：
Le Roux, Pierre-Marie F.;Christen, Danilo;Patocchi, Andrea
包含 3 个图和 2 个表 摘要 在之前的研究中，对 Passe Crassane (PC) x Harrow Sweet (HS) 杂交的梨 F1 后代进行了 QTL 分析。鉴定了与火疫病抗性相关的四个基因组区域，包括位于连锁群（LG）上的两个主要QTL，即Harrow Sweet的2A和4（HS02A和HS04）。在本研究中，我们通过绘制来自苹果属或梨属的其他 SSR 位点，报告了 LG HS02A 和 HS02B 的组合为单个 LG。因此，我们可以精确地识别 LG HS02 上的一个主要 QTL。我们还通过将新的 SSR 标记添加到预先存在的 LG HS04 中，确认了 LG HS04 上的推定 QTL。基于Harrow Sweet谱系的SSR标记分析，推测主要的HS02 QTL源自品种Early Sweet，而HS04 QTL则从Harrow Sweet追溯到Bartlett。我们还描述了对来自 Angelys x Harrow Sweet 杂交的第二个 F1 后代中火疫病严重性性状的主要 HS02 QTL 的验证。
With 3 figures and 2 tables Abstract In a previous study, a QTL analysis was conducted on a pear F1 progeny derived from a cross Passe Crassane (PC) x Harrow Sweet (HS). Four genomic regions associated with fire blight resistance were identified, including two main QTL located on linkage groups (LGs), 2A and 4 of Harrow Sweet (HS02A and HS04). In the present study, we report the combination of LGs HS02A and HS02B into a single LG by mapping additional SSR loci from Malus or Pyrus spp. We could thereby precisely identify a single major QTL on LG HS02. We also confirm a putative QTL on LG HS04 by including new SSR markers to the pre-existing LG HS04. Based on SSR marker analysis of Harrow Sweet pedigree, the major HS02 QTL is presumed to originate from the cultivar Early Sweet, while the HS04 QTL was traced from Harrow Sweet back to Bartlett. We also describe the validation of the major HS02 QTL for the fire blight severity trait in a second F1 progeny derived from a cross Angelys x Harrow Sweet.