Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection.
Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection.
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DOI：
10.1093/jxb/ert185
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发表时间：
2013-08
影响因子：
6.9
通讯作者：
Mullineaux PM
中科院分区：
文献类型：
--
作者：
Bechtold U;Albihlal WS;Lawson T;Fryer MJ;Sparrow PA;Richard F;Persad R;Bowden L;Hickman R;Martin C;Beynon JL;Buchanan-Wollaston V;Baker NR;Morison JI;Schöffl F;Ott S;Mullineaux PM
热胁迫作物遭受脱水、生长抑制和随之而来的水分生产率下降，水分生产率是可收获产品的产量作为终生水消耗的函数，并且是与植物生长和发育相关的性状。热激转录因子（HSF）基因不仅与植物的耐热性有关，而且与植物的生长发育有关，因此可以影响植物的水分生产力。在此，证明了具有增加的HSFA 1b表达的拟南芥植物在水分充足和水分限制条件下显示出增加的水分生产力和收获指数。在非应激的HSFA 1b过表达（HSFA 1bOx）植物中，509个基因表现出改变的表达，这些基因并没有过度代表与发育相关的基因，但对生物胁迫的反应。这证实了HSFA 1b在维持基础抗病性方面的额外作用，这是不依赖于应激激素的，但涉及H2 O2信号传导。509个基因中有55个在其启动子中含有热休克元件（HSE）的变体，这里命名为HSE 1b。染色质免疫沉淀-PCR证实HSFA 1b结合HSE 1b在体内，包括在7个转录因子基因。多蛋白桥接因子1c（MBF 1c）。植物过表达MBF 1c表现出增强的基础阻力，但不水分生产力，从而部分phenocopying HSFA 1bOx植物。对HSFA 1b和MBF 1c过表达的基因的比较揭示了一个共同的组，其中没有一个含有HSE 1b基序。从这个例子中，它表明，HSFA 1b直接调节55 HSE 1b含有基因，控制其余454个基因，共同占应激防御和发育表型的HSFA 1bOx。
Heat-stressed crops suffer dehydration, depressed growth, and a consequent decline in water productivity, which is the yield of harvestable product as a function of lifetime water consumption and is a trait associated with plant growth and development. Heat shock transcription factor (HSF) genes have been implicated not only in thermotolerance but also in plant growth and development, and therefore could influence water productivity. Here it is demonstrated that Arabidopsis thaliana plants with increased HSFA1b expression showed increased water productivity and harvest index under water-replete and water-limiting conditions. In non-stressed HSFA1b-overexpressing (HSFA1bOx) plants, 509 genes showed altered expression, and these genes were not over-represented for development-associated genes but were for response to biotic stress. This confirmed an additional role for HSFA1b in maintaining basal disease resistance, which was stress hormone independent but involved H2O2 signalling. Fifty-five of the 509 genes harbour a variant of the heat shock element (HSE) in their promoters, here named HSE1b. Chromatin immunoprecipitation-PCR confirmed binding of HSFA1b to HSE1b in vivo, including in seven transcription factor genes. One of these is MULTIPROTEIN BRIDGING FACTOR1c (MBF1c). Plants overexpressing MBF1c showed enhanced basal resistance but not water productivity, thus partially phenocopying HSFA1bOx plants. A comparison of genes responsive to HSFA1b and MBF1c overexpression revealed a common group, none of which harbours a HSE1b motif. From this example, it is suggested that HSFA1b directly regulates 55 HSE1b-containing genes, which control the remaining 454 genes, collectively accounting for the stress defence and developmental phenotypes of HSFA1bOx.
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