Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions
Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions
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DOI：
10.1007/s00253-003-1369-5
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发表时间：
2003-12-01
影响因子：
5
通讯作者：
Xu, P
中科院分区：
文献类型：
--
作者：
Chen, X;Zhang, DJ;Xu, P
研究了微氧条件下克雷伯氏菌发酵生产1，3-丙二醇（1，3-PD）的条件。分批发酵的实验结果表明，在微氧条件下的最终浓度和产率的1，3-PD的甘油接近厌氧条件下实现的值。然而，在发酵结束时，在微好氧条件下比厌氧条件下产生较少的乙醇。间歇式微好氧发酵时间明显短于厌氧发酵。这导致1，3-PD的生产率增加。以K. pneumoniae DSM 2026在发酵时间为6 h、甘油初始浓度为40 g/l时的产率分别为17.65 g/l、56.13%和2.94 g l（-1）h（-1）。与DSM 2026相比，K. pneumoniae AS 1.1736在相同条件下生长缓慢，1，3-PD浓度低。此外，还研究了K. pneumoniae DSM 2026在微好氧条件下比厌氧条件下更快。在微氧条件下分批补料发酵中1，3-PD的浓度、摩尔产率和生产率分别为59.50 g/l、51.75%和1.57 g l（-1）h（-1）。微氧条件下的体积生产率的1，3-PD几乎是厌氧补料分批发酵的两倍，分别为1.57和0.80克升（-1）小时（-1）。
The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under micro-aerobic conditions was investigated in this study. The experimental results of batch fermentation showed that the final concentration and yield of 1,3-PD on glycerol under micro-aerobic conditions approached values achieved under anaerobic conditions. However, less ethanol was produced under microaerobic than anaerobic conditions at the end of fermentation. The batch micro-aerobic fermentation time was markedly shorter than that of anaerobic fermentation. This led to an increment of productivity of 1,3-PD. For instance, the concentration, molar yield, and productivity of 1,3-PD of batch micro-aerobic fermentation by K. pneumoniae DSM 2026 were 17.65 g/l, 56.13%, and 2.94 g l(-1) h(-1), respectively, with a fermentation time of 6 h and an initial glycerol concentration of 40 g/l. Compared with DSM 2026, the microbial growth of K. pneumoniae AS 1.1736 was slow and the concentration of 1,3-PD was low under the same conditions. Furthermore, the microbial growth in fed-batch fermentation by K. pneumoniae DSM 2026 was faster under micro-aerobic than anaerobic conditions. The concentration, molar yield, and productivity of 1,3-PD in fed-batch fermentation under micro-aerobic conditions were 59.50 g/l, 51.75%, and 1.57 g l(-1) h(-1), respectively. The volumetric productivity of 1,3-PD under microaerobic conditions was almost twice that of anaerobic fed-batch fermentation, at 1.57 and 0.80 g l(-1) h(-1), respectively.