Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization.
Isolation of marine benzo[a]pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization.
复制标题
DOI：
10.1016/s1001-0742(08)62438-9
复制
发表时间：
2009
影响因子：
6.9
通讯作者：
Yi-Rui Wu;Teng-Teng He-Teng;Mingqi Zhong;Yueling Zhang;Enmin Li;Tongwang Huang;Zhong Hu
中科院分区：
文献类型：
--
作者：
Yi-Rui Wu;Teng-Teng He-Teng;Mingqi Zhong;Yueling Zhang;Enmin Li;Tongwang Huang;Zhong Hu
从中国厦门西海海域沉积物中分离得到一株对苯并[a]芘（BaP）具有较高降解能力的菌株BAP5，并鉴定为苍白杆菌（Ochrobactrum sp.）。根据16S rRNA基因序列以及Biolog微生物鉴定系统。菌株BAP5可以在含有50 mg/L BaP的矿物盐培养基中生长，培养30 d后降解约20% BaP。苍白杆菌属BAP5能够利用其他多环芳烃（PAHs）（如菲、芘和荧蒽）作为唯一的碳源和能源，表明其在PAHs生物修复中的潜在应用。苍白杆菌总可溶性蛋白的概况。还研究了 BAP5。通过二维聚丙烯酰胺凝胶电泳检测到菌株 BAP5 在 BaP 存在下孵育时的一些过度表达和特殊表达的蛋白质，并通过基质辅助激光解吸/电离飞行时间质谱进行肽指纹分析，发现这些蛋白质与 PAHs 代谢、DNA 翻译和能量产生相关。
A bacterial strain BAP5 with a relatively high degradation ability of benzo[a]pyrene (BaP) was isolated from marine sediments of Xiamen Western Sea, China and identified as Ochrobactrum sp. according to 16S rRNA gene sequence as well as Biolog microbial identification system. Strain BAP5 could grow in mineral salt medium with 50 mg/L of BaP and degrade about 20% BaP after 30 d of incubation. Ochrobactrum sp. BAP5 was able to utilize other polycyclic aromatic hydrocarbons (PAHs) (such as phenanthrene, pyrene and fluoranthene) as the sole carbon source and energy source, suggesting its potential application in PAHs bioremediation. The profile of total soluble protein from Ochrobactrum sp. BAP5 was also investigated. Some over- and special-expressed proteins of strain BAP5 when incubated with the presence of BaP were detected by two-dimensional polyacrylamide gel electrophoresis, and found to be related with PAHs metabolism, DNA translation, and energy production based on peptide fingerprint analysis through matrix-assisted laser desorption/ionization-time of flight mass spectrometry.