TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro
TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro
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DOI：
10.1016/s0142-9612(99)00077-0
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发表时间：
1999-10-01
期刊：
影响因子：
14
通讯作者：
van Luyn, MJA
中科院分区：
文献类型：
--
作者：
Khouw, IMSL;van Wachem, PB;van Luyn, MJA
成纤维细胞和肌成纤维细胞参与异物对生物材料的反应，尤其是包膜的形成。然而，胶囊的收缩或脱离可能会导致并发症。生物材料的生物相容性可以通过应用调节(肌)成纤维细胞分化或激活的蛋白质来改善。肌成纤维细胞区别于成纤维细胞，可以通过α-平滑肌肌动蛋白(α-SM肌动蛋白)的表达来识别。本实验研究了猪真皮细胞增殖和静止对细胞分化的影响，以及转化生长因子-β(TGF-β)和碱性成纤维细胞生长因子(BFGF)是否参与增殖细胞的分化。猪细胞之所以被使用，是因为猪越来越像活体模型一样发挥作用，而人们对它们的细胞特征知之甚少。无血清培养的静止成纤维细胞分化为肌成纤维细胞，而增殖的成纤维细胞在含转化生长因子-β的血清存在下培养，形成α-SM肌动蛋白阴性细胞团。在达到融合后，这些簇开始表达α-SM肌动蛋白。此外，这些增殖细胞从第4天开始产生转化生长因子-β，而碱性成纤维细胞生长因子则不产生。碱性成纤维细胞生长因子抑制成肌成纤维细胞的分化，而抗转化生长因子-β抗体的抑制作用更大。此外，还将从两种生物材料应用的角度讨论有关肌成纤维细胞在组织收缩中的作用的两种理论。(C)1999爱思唯尔科学有限公司。保留所有权利。
Fibroblasts and myofibroblasts are involved in the foreign body reaction to biomaterials, especially in capsule formation. However, contraction or detachment of the capsule can lead to complications. Biocompatibility of biomaterials may be improved by the application of proteins regulating the differentiation or activation of(myo)fibroblasts. Myofibroblasts, differentiating from fibroblasts can be identified by the expression of alpha-smooth muscle actin (alpha-SM actin). We investigated the influence of proliferation and quiescence on the differentiation of porcine dermal cells and whether transforming growth factor-beta (TGF-beta) and basic fibroblast growth factor (bFGF) are involved in the differentiation of proliferating cells. Porcine cells were used because pigs increasingly function as in vivo models while little is known of the characteristics of their cells. Serum-free cultured, quiescent fibroblasts differentiated into myofibroblasts, while proliferating fibroblasts cultured in the presence of serum containing TGF-beta, formed alpha-SM actin-negative cell clusters. After reaching confluency, these clusters started to expressing alpha-SM actin. Moreover, these proliferating cells produced TGF-beta from day 4 onwards while bFGF did not. Differentiation into myofibroblasts was inhibited by bFGF and to an even greater extent by antibodies to TGF-beta. Further, two theories concerning the role of the myofibroblast in tissue contraction in view of two biomaterial application will be discussed. (C) 1999 Elsevier Science Ltd. All rights reserved.