Kinetics of proton exchange of phosphatidylethanolamine in phospholipid vesicles.
Kinetics of proton exchange of phosphatidylethanolamine in phospholipid vesicles.
复制标题
磷脂囊泡中磷脂酰乙醇胺的质子交换动力学。
DOI：
10.1016/s0006-3495(85)83868-6
复制
发表时间：
1985
影响因子：
3.4
通讯作者：
Redfield,AG
中科院分区：
文献类型：
--
作者：
Ralph,EK;Lange,Y;Redfield,AG
用核磁共振波谱测定了磷脂酰乙醇胺(PE)在超声作用下混合磷脂囊泡中氨基质子的交换速率。交换速率随pH和磷酸盐浓度的增加而增加。在没有缓冲液的情况下，主要的交换过程是NH2基团通过水与外表面的NH3+基团反应的表面内反应。胆固醇的加入降低了表面内交换的速率常数。这些实验证明，这种反应可能在质子在膜表面内和向膜表面的传输中占主导地位。
The rate of proton exchange of the amino protons of phosphatidylethanolamine (PE) in sonicated mixed phospholipid vesicles has been determined by NMR spectroscopy. The rate of exchange increases with increasing pH and phosphate concentration. In the absence of buffer the dominant exchange process is an intrasurface reaction in which NH2 groups react via water with NH3+ groups on the outer surface. Addition of cholesterol reduces the rate constant for intrasurface exchange. The experiments are evidence that such reactions could be dominant in proton transport in and to membrane surfaces.