Determination of clovoxamine concentration in human plasma by electron capture gas chromatography.
Determination of clovoxamine concentration in human plasma by electron capture gas chromatography.
复制标题
电子捕获气相色谱法测定人血浆中的克洛沙明浓度。
作者：
H. Hurst;D. Jones;C. Jarboe;H. deBree
抗抑郁药氯伏沙明可通过电子捕获气相色谱法在人血浆中特异性和灵敏度定量。将氯伏沙明和内标物氟伏沙明在pH 12下从血浆中萃取到乙酸乙酯中，反萃取到磷酸中，并在90 ℃下水解形成酮衍生物，然后将其再萃取到己烷中以注入色谱仪中。可以测量每升血浆中低至1微克的氯伏沙明。在10和100 μ g/L浓度下，分析的变异系数（CV）分别为：批内，5.4%和2.7%;批间，17.5%和7.0%。当将该测定应用于参与氯伏沙明早期临床试验的个体的血浆时，在口服50 mg富马酸氯伏沙明后3小时，稳态血浆浓度范围为50至77 μ g/L。
The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90 degrees C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 microgram of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for th analysis at concentrations of 10 and 100 micrograms/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 micrograms/L 3 h after a 50-mg oral dose of clovoxamine fumarate.