Is Overoxidation of Peroxiredoxin Physiologically Significant?
Is Overoxidation of Peroxiredoxin Physiologically Significant?
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DOI：
10.1089/ars.2010.3717
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发表时间：
2011-02-15
影响因子：
6.6
通讯作者：
Jakob, Ursula
中科院分区：
文献类型：
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作者：
Thamsen, Maike;Kumsta, Caroline;Jakob, Ursula
真核生物的过氧化物氧化还原酶对亚磺酸的形成非常敏感。这种过度氧化被认为是将过氧化物酶转化为分子伴侣，可以被硫氧还蛋白逆转。一些生物，包括秀丽隐杆线虫，缺乏sulfiredoxins，但编码sestrins，蛋白质被认为是功能等同的。我们在C.过氧化氢脉冲很短的线虫我们发现，过氧化的过氧化物酶2（PRDX-2）的还原是非常缓慢和sestrin独立的，强烈暗示蠕虫缺乏一个有效的修复系统。分析了PRDX-2在C. elegans寿命显示在任何点都没有过氧化的PRDX-2的积累，质疑蠕虫中的PRDX-2过氧化是否与生理学相关。抗氧化剂。氧化还原信号。14，725-730。
Eukaryotic peroxiredoxins are highly susceptible to sulfinic acid formation. This overoxidation, which is thought to convert peroxiredoxins into chaperones, can be reversed by sulfiredoxins. Several organisms, including Caenorhabditis elegans, lack sulfiredoxins but encode sestrins, proteins proposed to be functionally equivalent. We induced peroxiredoxin overoxidation in C. elegans with a short peroxide pulse. We found that reduction of overoxidized peroxiredoxin 2 (PRDX-2) was extremely slow and sestrin-independent, strongly implying that worms lack an efficient repair system. Analysis of PRDX-2's overoxidation status during C. elegans lifespan revealed no accumulation of overoxidized PRDX-2 at any point, questioning whether PRDX-2 overoxidation in worms is physiologically relevant. Antioxid. Redox Signal. 14, 725-730.