Nocardioides szechwanensis sp. nov. and Nocardioides psychrotolerans sp. nov., isolated from a glacier.
Nocardioides szechwanensis sp. nov. and Nocardioides psychrotolerans sp. nov., isolated from a glacier.
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DOI：
10.1099/ijs.0.038091-0
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发表时间：
2013
影响因子：
2.8
通讯作者：
Qing Liu;Yuhua Xin;Hong-Can Liu;Yuguang Zhou;Y. Wen
中科院分区：
文献类型：
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作者：
Qing Liu;Yuhua Xin;Hong-Can Liu;Yuguang Zhou;Y. Wen
从中国四川省海螺沟冰川中分离出两种革兰氏阳性、杆状、不形成芽孢的细菌（菌株 RHLT(1)-17(T) 和 RHLT(2)-1(T)）。基于16S rRNA基因序列的系统发育分析表明，该菌株属于诺卡氏菌属，彼此之间具有97.8%的序列相似性，与诺卡氏菌KSL-2(T)的序列相似性分别为97.6%和98.4%。菌株RHLT(1)-17(T)在0-35℃下生长，菌株RHLT(2)-1(T)在0-25℃下生长。菌株RHLT(1)-17(T)的主要细胞脂肪酸为C(17:1)ω8c(32.69%)和iso-C(16:0)(21.74%)。菌株 RHLT(2)-1(T) 的主要细胞脂肪酸为 C(18:1)ω9c (28.72%)，总特征 3 (17.14%；包含 C(16:1)ω7c 和/或 C(16:1)ω6c)、iso-C(16:0) (14.35%)、C(16:0) (9.96%) 和异-C(14:0) (8.34%)。两种菌株的细胞壁肽聚糖中都含有11-2,6-二氨基庚二酸，并且MK-8(H(4))作为主要的甲萘醌。根据使用多相方法获得的数据，两个新物种，诺卡氏菌（Nocardioides szechwanensis sp.）。十一月（类型菌株 RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)）和诺卡氏菌 psychrotolerans sp。十一月（类型应变 RHLT(2)-1(T) = CGMCC 1.11156(T) = NBRC 108563(T)），建议。
Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT(1)-17(T) and RHLT(2)-1(T)) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with Nocardioides kribbensis KSL-2(T). Strain RHLT(1)-17(T) grew at 0-35 °C and strain RHLT(2)-1(T) grew at 0-25 °C. The major cellular fatty acids of strain RHLT(1)-17(T) were C(17 : 1)ω8c (32.69 %) and iso-C(16 : 0) (21.74 %). The major cellular fatty acids of strain RHLT(2)-1(T) were C(18 : 1)ω9c (28.72 %), summed feature 3 (17.14 %; comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), iso-C(16 : 0) (14.35 %), C(16 : 0) (9.96 %) and iso-C(14 : 0) (8.34 %). Both strains contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H(4)) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)) and Nocardioides psychrotolerans sp. nov. (type strain RHLT(2)-1(T) =CGMCC 1.11156(T) = NBRC 108563(T)), are proposed.