How to impose microscopic: Reversibility in complex reaction mechanisms
How to impose microscopic: Reversibility in complex reaction mechanisms
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DOI：
10.1529/biophysj.103.038679
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发表时间：
2004-06-01
影响因子：
3.4
通讯作者：
Plested, AJR
中科院分区：
文献类型：
--
作者：
Colquhoun, D;Dowsland, KA;Plested, AJR
大多数（但不是全部）离子通道似乎遵循微观可逆性（或详细平衡）定律。因此，在反应机制的拟合过程中，通常要求机制中的循环始终遵循微观可逆性。在复杂的反应机制中，尤其是那些包含立方态排列的反应机制中，如何实现这一点可能并不明显。描述了施加微观可逆性的三种通用方法。第一种方法的工作原理是按正确的顺序设置“明显”的四状态循环。第二种方法基于生成树的思想，通过查找独立的循环（通常具有四个以上的状态）来工作，这样它们的设置顺序并不重要。第三种方法使用线性代数来求解约束速率。
Most, but not all, ion channels appear to obey the law of microscopic reversibility (or detailed balance). During the fitting of reaction mechanisms it is therefore often required that cycles in the mechanism should obey microscopic reversibility at all times. In complex reaction mechanisms, especially those that contain cubic arrangements of states, it may not be obvious how to achieve this. Three general methods for imposing microscopic reversibility are described. The first method works by setting the 'obvious' four-state cycles in the correct order. The second method, based on the idea of a spanning tree, works by finding independent cycles (which will often have more than four states) such that the order in which they are set does not matter. The third method uses linear algebra to solve for constrained rates.