Epidermal growth factor modulates thyroid growth and function in culture.
Epidermal growth factor modulates thyroid growth and function in culture.
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表皮生长因子在培养物中调节甲状腺生长和功能。
DOI：
10.1210/endo-112-5-1680
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
B. Westermark
中科院分区：
文献类型：
--
作者：
K. Westermark;F. Karlsson;B. Westermark
研究了表皮生长因子（EGF）对猪甲状腺滤泡悬浮培养物生长和功能的影响。当与EGF （0.01-10 ng/ml）孵育时，显著刺激[3H]胸苷的掺入，在0.5 ng/ml左右达到半最大值。通过6天的实验，[3H]胸苷的掺入在初始滞后期1天后几乎呈线性增加。放射自显影分析显示，EGF （10 ng/ml）孵育4天后，超过50%的细胞核被标记，而对照组的标记细胞核为3-7%。EGF在无血清培养基中与在含有1%胎牛血清的培养基中同样有效。在相同浓度下添加EGF，刺激[3H]胸腺嘧啶的掺入，发现在添加后30分钟内降低卵泡的碘代谢；EGF的加入抑制了tsh刺激的外排和[125I]碘化物的组织。由于EGF是一种生理上发生的物质，数据表明EGF可能是体内甲状腺生长和功能的调节剂。
The effect of epidermal growth factor (EGF) on the growth and function of porcine thyroid follicle suspension cultures was examined. When incubated with EGF (0.01-10 ng/ml), the incorporation of [3H]thymidine was markedly stimulated, with the half-maximal effect occurring at about 0.5 ng/ml. Through 6-day experiments, [3H]thymidine incorporation increased almost linearly after an initial lag phase of 1 day. Autoradiographical analysis showed that more than 50% of the nuclei were labeled after 4 days of incubation with EGF (10 ng/ml) compared to control values of 3-7% labeled nuclei. EGF was essentially as effective in serum-free medium as in medium containing 1% fetal calf serum. EGF added at the same concentrations that stimulated incorporation of [3H]thymidine was found to reduce iodide metabolism of the follicles within 30 min of addition; both TSH-stimulated efflux and organification of [125I]iodide were suppressed by the addition of EGF. Since EGF is a physiologically occurring substance, the data suggest that EGF might be a modulator of thyroid growth and function in vivo.