Poly(L-glutamic acid) Grafted with Oligo(2-(2-(2-methoxyethoxy)ethoxy) ethyl methacrylate): Thermal Phase Transition, Secondary Structure, and Self-Assembly
Poly(L-glutamic acid) Grafted with Oligo(2-(2-(2-methoxyethoxy)ethoxy) ethyl methacrylate): Thermal Phase Transition, Secondary Structure, and Self-Assembly
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聚（L-谷氨酸）接枝低聚（2-（2-（2-甲氧基乙氧基）乙氧基）甲基丙烯酸乙酯）：热相变、二级结构和自组装
DOI：
10.1002/pola.24698
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发表时间：
2011-06-15
影响因子：
--
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Ding, Jianxun;Xiao, Chunsheng;Chen, Xuesi
温度响应性和pH响应性接枝共聚物，聚（L-谷氨酸）-g-oligo（2-（2-（2-甲氧基乙氧基）乙氧基）乙基甲基丙烯酸酯）和聚（L-谷氨酸-共-（L-谷氨酸-g-寡聚（2-（2-（2-甲氧基乙氧基）乙氧基）乙基甲基丙烯酸酯），通过N-羧酸酐（NCA）单体的开环聚合（ROP）和随后的2-羧基-N-羧酸酐（NCA）的原子转移自由基聚合（ATRP）合成了N-羧酸酐（NCA），甲基丙烯酸（2-（2-甲氧基乙氧基）乙氧基）乙酯。接枝共聚物的热响应性可以通过低聚甲基丙烯酸2-（2-（2-甲氧基乙氧基）乙氧基）乙酯（奥梅奥（3）MA）的分子量、聚谷氨酸（PLGA）主链的组成和水溶液的pH值来调节。接枝共聚物的α-螺旋含量受奥梅奥（3）MA长度和水溶液pH值的影响。此外，接枝共聚物表现出可调的自组装行为。胶束的流体动力学半径（R-h）和临界胶束化浓度值与奥梅奥（3）MA的长度和可生物降解的PLGA骨架的组成有关。R-h也可以通过温度和pH值来调节。最后，在体外的甲基噻唑基四唑（MTT）测定表明，接枝共聚物是生物相容性的HeLa细胞。因此，这些接枝共聚物具有良好的生物相容性，明确的二级结构，以及单，双响应性，是很有前途的刺激响应材料的生物医学应用。(C)2011 Wiley Periodicals，Inc.聚合物科学杂志A部分：聚合物化学49：2665-2676，2011
Thermoresponsive and pH-responsive graft copolymers, poly(L-glutamate)-g-oligo(2-(2-(2-methoxyethoxy) ethoxy) ethyl methacrylate) and poly(L-glutamic acid-co-(L-glutamate-g-oligo(2-(2-(2-methoxyethoxy) ethoxy) ethyl methacrylate))), were synthesized by ring-opening polymerization (ROP) of N-carboxyanhydride (NCA) monomers and subsequent atom transfer radical polymerization of 2-(2-(2-methoxyethoxy) ethoxy) ethyl methacrylate. The thermoresponsiveness of graft copolymers could be tuned by the molecular weight of oligo(2-(2-(2-methoxyethoxy) ethoxy) ethyl methacrylate) (OMEO(3)MA), composition of poly(L-glutamic acid) (PLGA) backbone and pH of the aqueous solution. The alpha-helical contents of graft copolymers could be influenced by OMEO(3)MA length and pH of the aqueous solution. In addition, the graft copolymers exhibited tunable self-assembly behavior. The hydrodynamic radius (R-h) and critical micellization concentration values of micelles were relevant to the length of OMEO(3)MA and the composition of biodegradable PLGA backbone. The R-h could also be adjusted by the temperature and pH values. Lastly, in vitro methyl thiazolyl tetrazolium (MTT) assay revealed that the graft copolymers were biocompatible to HeLa cells. Therefore, with good biocompatibility, well-defined secondary structure, and mono-, dual-responsiveness, these graft copolymers are promising stimuli-responsive materials for biomedical applications. (C) 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 49: 2665-2676, 2011