MULTI-FLORET SPIKELET 4 (MFS4) Regulates Spikelet Development and Grain Size in Rice
MULTI-FLORET SPIKELET 4 (MFS4) Regulates Spikelet Development and Grain Size in Rice
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DOI：
10.1016/j.rsci.2021.05.005
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发表时间：
2021-07
期刊：
影响因子：
4.8
通讯作者：
Wang Yan;Zeng Xiaoqin;Lu Lu-Lu;Q. Cheng;Yang Fayu;Mingjiang Huang;X. Mao;Yunfeng Li
中科院分区：
文献类型：
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作者：
Wang Yan;Zeng Xiaoqin;Lu Lu-Lu;Q. Cheng;Yang Fayu;Mingjiang Huang;X. Mao;Yunfeng Li
小穗是水稻花序的基本单位，其发育对水稻产量和品质有重要影响。我们报道了一个水稻小穗多花小穗4号（mfs 4），它产生额外的花器官或一个完整的额外小花，并延长不育外稃。结果表明，MFS 4基因的突变干扰了小穗分生组织的决定性和花器官的同一性。此外，突变体的株高和籽粒长、宽均小于野生型。用混合分离分析法将MFS 4基因定位在第1号染色体长臂上一个557 kb的区域。序列分析表明，LOC_Os01 g67430的开放阅读框有一个C碱基缺失。进一步的检测表明，LOC_Os01g67430的野生型拷贝能够逆转其fs 4缺陷，这表明LOC_Os01g67430是MFS 4基因。MFS 4基因编码位于线粒体中的脂肪酶，在幼花序中强烈表达。qRT-PCR结果表明，在突变体fs 4中，一些已知的小穗分生组织决定性和籽粒大小调控基因的表达量降低，表明MFS 4基因通过调控这些基因的表达来调控小穗分生组织决定性和籽粒大小。
In rice, the spikelet is the basic unit of inflorescence, and its development is important for determining the grain yield and quality. We reported a rice spikelet mutantmulti-floret spikelet 4(mfs4) which resulted in the production of extra floral organs or a whole extra floret, and elongated sterile lemmas. The results suggested that the mutation of theMFS4gene interfered with spikelet meristem determinacy and floral organ identity. In addition, the plant height and the grain length and width in themfs4mutant were all less than those in the wild type. Using the bulked segregant analysis method, theMFS4gene was localized in a 557-kb region on the long arm of chromosome 1. Sequence analysis showed that there was a C-base deletion at the open reading frame ofLOC_Os01g67430. Further tests indicated that a wild type copy ofLOC_Os01g67430was able to reverse themfs4defects, which indicated thatLOC_Os01g67430was theMFS4gene. TheMFS4gene encodes a lipase located in the mitochondria and is expressed strongly in the young inflorescence. qRT-PCR results showed that the expression of some genes that were known to regulate spikelet meristem determinacy and grain size were decreased in themfs4mutant, which indicated that theMFS4gene regulates spikelet meristem determinacy and grain size by modulating the expression of these genes.