Localization of Liv2 as an immature hepatocyte marker in EB outgrowth.
Localization of Liv2 as an immature hepatocyte marker in EB outgrowth.
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DOI：
10.1100/tsw.2009.18
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发表时间：
2009-03-01
影响因子：
--
通讯作者：
Sasaki K
中科院分区：
文献类型：
--
作者：
Takashimizu I;Tanaka Y;Yoshie S;Kano Y;Ichikawa H;Cui L;Ogiwara N;Johkura K;Sasaki K
本研究的目的是通过获得正常小鼠胚胎中Liv 2的基本数据，并通过确认小鼠ES衍生细胞中Liv 2的表达，将小鼠未成熟肝细胞（成肝细胞）的表面标志物Liv 2确立为胚胎干（ES）细胞衍生的未成熟肝细胞的选择工具。Liv 2的估计分子量为40-45 kDa，在胚胎第9.5天（E）在胎肝细胞的细胞膜中明确检测到免疫反应性，逐渐下降，直到E12.5，随后变得不可检测。Liv 2定位于细胞膜上并靠近细胞膜。小鼠ES细胞形成拟胚体（EB），悬滴法Nanog免疫染色证实其未分化状态。少数Liv 2阳性细胞在第7天在EB生长中以簇的形式出现，但其中只有一些在第13天是白蛋白（ALB）阳性的。这些细胞具有相同的免疫反应性模式，即，在细胞膜上定位，如发育中的肝脏中的未成熟肝细胞，尽管存在具有不同免疫反应性模式的其他类型的细胞，其仅在细胞质中被视为颗粒模式，并且没有ALB或神经元标记物巢蛋白。这些结果表明，Liv 2可能是有用的作为一个表面标记的未成熟肝细胞来源于ES细胞。这种应用将允许对未成熟肝细胞的唯一选择，并为再生医学提供有用的工具。
The objective of this study was to establish Liv2, a surface marker of mouse immature hepatocytes (hepatoblasts), as a selection tool for embryonic stem (ES) cell–derived immature hepatocytes by acquiring basic data on Liv2 in normal mouse embryos and by confirming Liv2 expression in mouse ES-derived cells. The estimated molecular weight of Liv2 was 40–45 kDa, and immunoreactivity was definitively detected in the cell membrane of fetal hepatocytes on embryonic day (E) 9.5, declined gradually until E12.5, and subsequently became undetectable. Liv2 was localized on and close to the cell membrane. Embryoid bodies (EB) were formed from mouse ES cells whose undifferentiated state was confirmed with immunostaining of Nanog by the hanging drop method. A few Liv2-positive cells occurred as a cluster in EB outgrowth on day 7, but only some of these were albumin (ALB)-positive on day 13. These cells had the same pattern of immunoreactivity, i.e., localization on the cell membrane, as immature hepatocytes in the developing liver, although there were other types of cells with a different pattern of immunoreactivity that were seen only as a granular pattern in the cytoplasm and without ALB or the neuronal marker nestin. These results suggest that Liv2 may be useful as a surface marker for immature hepatocytes derived from ES cells. This application would allow for the sole selection of immature hepatocytes and provide a useful tool for regenerative medicine.
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影响因子：
13.5
作者：
Suzuki, A;Zheng, YW;Taniguchi, H
通讯作者：
Taniguchi, H
DOI：
10.1016/j.jsgi.2005.01.004
发表时间：
2005-05-01
期刊：
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
影响因子：
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作者：
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通讯作者：
Sasaki, K
影响因子：
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作者：
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通讯作者：
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影响因子：
5.2
作者：
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通讯作者：
Sasaki, Katsunori
DOI：
10.1152/ajprenal.00001.2004
发表时间：
2006-01-01
影响因子：
4.2
作者：
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通讯作者：
Sasaki, K