Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model
Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model
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DOI：
10.1016/j.biomaterials.2013.07.065
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发表时间：
2013-12-01
期刊：
影响因子：
14
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Kobayashi, Toyoshige;Kan, Kazutoshi;Okano, Teruo
我们已经在几个领域对人类患者进行了基于细胞片的再生医学的临床应用。为了实现可移植细胞片的大规模生产，我们开发了自动化细胞培养系统。在这里，我们报告了一个自动化的机器人系统，利用细胞培养容器，细胞盒。细胞盒有两个房间的上皮细胞和饲养层细胞分离的多孔膜上的温度响应性聚合物共价固定。在将细胞倒入该机器人系统后，根据计算机程序自动进行培养期间的细胞接种、培养基更换和显微镜检查。可移植的角膜上皮细胞片成功地制造在细胞盒与这个机器人系统。然后，将制造的细胞片移植到兔角膜缘上皮干细胞缺陷模型的眼表面上，在6小时运输后，使用便携式恒温容器将内部温度保持在36 ℃。移植后1周内，正常角膜上皮成功再生。该自动化细胞培养系统将有助于再生医学组织工程产品的产业化。(C)2013爱思唯尔有限公司保留所有权利。
We have performed clinical applications of cell sheet-based regenerative medicine with human patients in several fields. In order to achieve the mass production of transplantable cell sheets, we have developed automated cell culture systems. Here, we report an automated robotic system utilizing a cell culture vessel, cell cartridge. The cell cartridge had two rooms for epithelial cells and feeder layer cells separating by porous membrane on which a temperature-responsive polymer was covalently immobilized. After pouring cells into this robotic system, cell seeding, medium change, and microscopic examination during culture were automatically performed according to the computer program. Transplantable corneal epithelial cell sheets were successfully fabricated in cell cartridges with this robotic system. Then, fabricated cell sheets were transplanted onto ocular surfaces of rabbit limbal epithelial stem cell deficiency model after 6-h transportation using a portable homothermal container to keep inner temperature at 36 degrees C. Within one week after transplantation, normal corneal epithelium was successfully regenerated. This automatic cell culture system would be useful for industrialization of tissue-engineered products for regenerative medicine. (C) 2013 Elsevier Ltd. All rights reserved.