Recyclable beta-Glucosidase by One-Pot Encapsulation with Cu-MOFs for Enhanced Hydrolysis of Cellulose to Glucose
Recyclable beta-Glucosidase by One-Pot Encapsulation with Cu-MOFs for Enhanced Hydrolysis of Cellulose to Glucose
复制标题
通过使用 Cu-MOFs 一锅封装可回收的 β-葡萄糖苷酶，以增强纤维素水解为葡萄糖
DOI：
10.1021/acssuschemeng.8b05489
复制
发表时间：
2019
影响因子：
8.4
通讯作者：
Wang Yun
中科院分区：
文献类型：
--
作者：
Wang Lei;Zhi Wenjing;Wan Jing;Han Juan;Li Chunmei;Wang Yun
最近的研究证明，通过将酶封装在称为金属有机框架（MOF）的强大多孔材料中，可以提高酶的稳定性和可重复使用性。然而，在所有这些报告中，MOF 在酸性条件下的稳定性是在低 pH 下具有催化活性的酶的主要问题。本研究的目的是利用共沉淀方法将 β-葡萄糖苷酶 (β-G) 封装到 Cu-MOF 中，因为它具有酸性稳定性。事实上，所制备的具有高封装效率的β-G@Cu(PABA)生物复合材料表现出增强的对酸性和热条件以及有机溶剂的耐受性。重要的是，β-G@Cu(PABA) 生物复合材料表现出优异的可重复使用性，即使在 10 个循环后仍保留 90% 的活性。以羧甲基纤维素（CMC）水解为模式，β-G@Cu(PABA)生物复合材料与共固定化纤维素酶的混合物提供了98%的葡萄糖产率，与单独共固定化纤维素相比提高了一倍。 β-G@Cu(PABA)生物复合材料和共固定化纤维素酶可以很容易地回收和再循环，在水解CMC的八个循环中仍然保持70%的生产率。这一发现首次凸显了 Cu-MOF 在酸性条件下保存酶的潜力。通过共沉淀方法开发的β-G@Cu(PABA)生物复合材料为基于酶的工业应用提供了有价值的平台。
Enhancing the stability and reusability of enzymes by encapsulating them within a powerful class of porous materials termed metal–organic frameworks (MOFs) has been demonstrated by recent studies. However, in all of these reports, the stability of MOFs under acidic conditions was a major issue for enzymes with catalytic activity at low pH. The objective of this study is to encapsulate the β-glucosidase (β-G) into the Cu-MOF due to its acidic stability, using a coprecipitation approach. Indeed, the as-prepared β-G@Cu(PABA) biocomposite with high encapsulation efficiency exhibited enhanced resistance to acidic and thermal conditions as well as organic solvents. Importantly, the β-G@Cu(PABA) biocomposite showed superior reusability, retaining 90% of activity even after 10 cycles. Using the hydrolysis of carboxymethylcellulose (CMC) as a mode, the mixture of β-G@Cu(PABA) biocomposite and coimmobilized cellulase afforded 98% glucose yield, which was twofold enhancement when compared with coimmobilized cellulose alone. The β-G@Cu(PABA) biocomposite and coimmobilized cellulase could been easily recovered and recycled to still retain 70% productivity in the eight cycles for hydrolyzing CMC. This discovery for the first time highlights the potential of Cu-MOF in preserving the enzymes under acidic conditions. The developed β-G@Cu(PABA) biocomposite by coprecipitation approach affords a valuable platform for enzyme-based industrial applications.