Diagnostic values of plasma matrix metalloproteinase-7, interleukin-8, and gamma-glutamyl transferase in biliary atresia
Diagnostic values of plasma matrix metalloproteinase-7, interleukin-8, and gamma-glutamyl transferase in biliary atresia
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DOI：
10.1007/s00431-022-04612-7
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发表时间：
2022-09-12
影响因子：
3.6
通讯作者：
Xiao, Yongtao
中科院分区：
文献类型：
--
作者：
Wu, Bo;Zhou, Ying;Xiao, Yongtao
胆道闭锁是一种严重的儿童胆汁淤积性肝病，以胆汁淤积和肝纤维化为特征。BA的早期诊断仍然具有挑战性。本研究旨在评价基质金属蛋白酶-7（MMP-7）、白细胞介素-8（IL-8）和γ-谷氨酰转移酶（GGT）在BA中的诊断价值。回顾性分析了2013年至2018年期间诊断为BA和非BA的婴儿。在这些婴儿中测量MMP-7、IL-8和GGT的血浆水平。采用受试者工作特征（ROC）曲线和ROC曲线下面积（AUC）评估MMP-7、IL-8和GGT的诊断价值。免疫荧光法检测肝组织中MMP-7和IL-8的表达。共有229名婴儿参加了这项研究，其中156名BA婴儿和73名非BA婴儿，包括16名婴儿肝炎综合征婴儿。BA婴儿的MMP-7、IL-8和GGT血浆水平中位数为11.8 ng/mL，（四分位距，IQR：5.3-57.5），1.5 ng/mL（IQR：1.0-2.8）和381.0 U/L（IQR：197.0-749.0），分别高于非BA受试者[MMP-7，4.4 ng/mL（IQR：3.3-6.1）; IL-8，0.7 ng/mL（IQR：0.5-1.0）; GGT，59.0 U/L（IQR：26.0-124.0）]。MMP-7、IL-8和GGT诊断BA的AUC值分别为0.8035、0.8083和0.9126。MMP-7 + IL-8、MMP-7 + GGT、IL-8 + GGT和MMP-7 + IL-8 + GGT诊断BA的AUC值分别为0.8248、0.9382、0.9168和0.9392。MMP-7、IL-8和GGT的AUC值用于区分有胆便的BA婴儿和有胆便的非BA婴儿，分别为0.8006、0.8258和0.9141。结论：血浆MMP-7、IL-8和GGT单独或联合检测对BA与非BA的鉴别诊断具有良好的准确性，可作为BA的可靠生物标志物。
Biliary atresia (BA) is a severe cholestatic liver disease in children featuring cholestasis and liver fibrosis. The early diagnosis of BA is still challenging. This study aimed to evaluate the diagnostic values of matrix metalloprotease-7 (MMP-7), interleukin-8 (IL-8), and gamma-glutamyl transferase (GGT) in BA. Infants diagnosed with BA and non-BA between 2013 and 2018 were retrospectively analyzed. Plasma levels of MMP-7, IL-8, and GGT were measured in these infants. The receiver operating characteristic (ROC) curves and area under the ROC curve (AUC) were used to assess the diagnostic values of MMP-7, IL-8, and GGT. The expression of MMP-7 and IL-8 in the livers was detected by immunofluorescence staining. A total of 229 infants were enrolled in this study: 156 BA infants and 73 non-BA infants including 16 ones with infantile hepatitis syndrome. The plasma levels of MMP-7, IL-8, and GGT in BA infants had a median of 11.8 ng/mL (interquartile range, IQR: 5.3-57.5), 1.5 ng/mL (IQR: 1.0-2.8), and 381.0 U/L (IQR: 197.0-749.0), respectively, which were higher than non-BA subjects [MMP-7, 4.4 ng/mL (IQR: 3.3-6.1); IL-8, 0.7 ng/mL (IQR: 0.5-1.0); GGT, 59.0 U/L (IQR: 26.0-124.0)]. The AUC values of MMP-7, IL-8, and GGT for the diagnosis of BA were 0.8035, 0.8083, and 0.9126, respectively. The AUC values of MMP-7 + IL-8, MMP-7 + GGT, IL-8 + GGT, and MMP-7 + IL-8 + GGT for the diagnosis of BA were 0.8248, 0.9382, 0.9168, and 0.9392, respectively. The AUC values of MMP-7, IL-8, and GGT for differentiating BA infants with cholic stool from non-BA infants with cholic stool were 0.8006, 0.8258, and 0.9141, respectively. The expression of MMP-7 and IL-8 was increased in the cholangiocytes in BA livers.Conclusion: Plasma MMP-7, IL-8, and GGT alone or a combination of them has good accuracy to differentiate BA from non-BA and may be reliable biomarkers for BA.