2-Aminoethoxydiphenyl borate (2-APB) reduces alkaline phosphatase release, CD63 expression, F-actin polymerization and chemotaxis without affecting the phagocytosis activity in bovine neutrophils
2-Aminoethoxydiphenyl borate (2-APB) reduces alkaline phosphatase release, CD63 expression, F-actin polymerization and chemotaxis without affecting the phagocytosis activity in bovine neutrophils
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DOI：
10.1016/j.vetimm.2011.12.006
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发表时间：
2012-01-15
影响因子：
1.8
通讯作者：
Burgos, R. A.
中科院分区：
文献类型：
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作者：
Conejeros, I.;Velasquez, Z. D.;Burgos, R. A.
2-氨基乙氧基二苯基硼酸酯 (2-APB) 干扰 Ca2+ 流入并减少牛中性粒细胞中 ROS 的产生、明胶酶的分泌和 CD11b 的表达。此外，有人认为，抑制参与钙库操作的 Ca2+ 进入 (SOCE) 的 Ca2+ 通道是开发牛新型抗炎药物的潜在目标，但尚不清楚 2-APB 是否影响与先天免疫反应相关的中性粒细胞功能。本研究描述了 2-APB（一种假定的 SOCE 抑制剂）对碱性磷酸酶活性（分泌囊泡标记物）、CD63（天青颗粒标记物）、F-肌动蛋白聚合以及血小板激活因子 (PAF) 刺激的牛中性粒细胞体外趋化性的影响。此外，我们还评估了 2-APB 对大肠杆菌和金黄色葡萄球菌生物颗粒吞噬活性的影响。我们观察到，2-APB >= 10 μM 的剂量显着降低碱性磷酸酶活性和体外趋化性，而 2-APB 50 >= μM 的浓度降低 CD63 表达和 F-肌动蛋白聚合。最后，我们观察到 2-APB 不影响与大肠杆菌和金黄色葡萄球菌生物颗粒一起孵育的中性粒细胞的吞噬活性。我们得出的结论是，抑制 Ca2+ 内流可能是减少牛炎症过程的有效策略。 (C) 2012 Elsevier B.V. 保留所有权利。
2-Aminoethoxydiphenyl borate (2-APB) interferes with the Ca2+ influx and reduces the ROS production, gelatinase secretion and CD11b expression in bovine neutrophils. Moreover, it has been suggested that inhibition of the Ca2+ channel involved in the store operated Ca2+ entry (SOCE) is a potential target for the development of new anti-inflammatory drugs in cattle, however it is unknown whether 2-APB affects neutrophil functions associated with the innate immune response. This study describes the effect of 2-APB, a putative SOCE inhibitor, on alkaline phosphatase activity a marker of secretory vesicles, CD63 a marker for azurophil granules, F-actin polymerization and in vitro chemotaxis in bovine neutrophils stimulated with platelet-activating factor (PAF). Also, we evaluated the effect of 2-APB in the phagocytic activity against Escherichia coli and Staphylococcus aureus bioparticles. We observed that doses of 2-APB >= 10 mu M significantly reduced alkaline phosphatase activity and in vitro chemotaxis, whereas concentrations of 2-APB 50 >= mu M reduced CD63 expression and F-actin polymerization. Finally, we observed that 2-APB did not affect the phagocytic activity in neutrophils incubated with E. coli and S. aureus bioparticles. We concluded that inhibition of Ca2+ influx could be a useful strategy to reduce inflammatory process in cattle. (C) 2012 Elsevier B.V. All rights reserved.