Differentiation of aspartic and isoaspartic acids using electron transfer dissociation
Differentiation of aspartic and isoaspartic acids using electron transfer dissociation
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DOI：
10.1016/j.jasms.2005.08.019
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
McLuckey, SA
中科院分区：
文献类型：
--
作者：
O'Connor, PB;Cournoyer, JJ;McLuckey, SA
电子转移解离允许使用先前通过电子捕获解离观察到的相同 c + 57 和 z - 57 峰来区分异天冬氨酸和天冬氨酸残基。这些峰清楚地定义了异天冬氨酸残基的存在和位置，并且它们相对丰富。离子阱仪器的较低分辨率使得检测天冬氨酸残基的诊断峰变得困难，因为精氨酸残基的侧链碎片离子会受到干扰，但在主链裂解中仍然可以清楚地观察到天冬氨酸残基，并且可以根据异天冬氨酸诊断离子的缺失来推断。
Electron-transfer dissociation allows differentiation of isoaspartic acid and aspartic acid residues using the same c + 57 and z - 57 peaks that were previously observed with electron capture dissociation. These peaks clearly define both the presence and the position of isoaspartic acid residues and they are relatively abundant. The lower resolution of the ion trap instrument makes detection of the aspartic acid residue's diagnostic peak difficult because of interference with side-chain fragment ions from arginine residues, but the aspartic acid residues are still clearly observed in the backbone cleavages and can be inferred from the absence of the isoaspartic acid diagnostic ions.