Development of fluorescence change-based, reagent-less optic immunosensor
Development of fluorescence change-based, reagent-less optic immunosensor
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DOI：
10.1016/j.bios.2004.06.041
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发表时间：
2005-02-15
影响因子：
12.6
通讯作者：
Kudo, M
中科院分区：
文献类型：
--
作者：
Aoyagi, S;Kudo, M
研制了一种免试剂、可再生、便携式光学免疫传感器。将荧光标记的蛋白A固定在荧光激发和荧光发射的光纤上，利用荧光强度的变化对免疫球蛋白G（IgG）进行检测。将带有QdotTM标记的蛋白A固定化玻璃板的光纤插入pH7.4的磷酸盐缓冲盐水溶液中。在荧光强度稳定后，加入IgG，并在与光纤连接的荧光计上测量荧光强度的时间过程。此外，用相同的系统评价了瞬态的荧光响应，当Qdot标记的蛋白A与IgG结合时，由于IgG的抑制，荧光强度降低。荧光降低的程度取决于稳态和瞬态的IgG浓度。(C)2004 Elsevier B.V.保留所有权利。
A reagent-less, regenerable and portable optic immunosensor was developed. A model sample, immunoglobulin G (IgG), was detected with this system based on changes in fluorescent intensity of fluorescent labeled protein A with specific reactivity to IgG depending on a reaction between the proteins.A glass plate immobilized with Qdot(TM)-labeled protein A was placed on the top of optic fibers designed for both excitation and fluorescence emission. The optic fibers with the Qdot(TM)-labeled protein A-immobilized glass plate were inserted into a solution of pH 7.4 phosphate buffered saline. After stabilization of the fluorescence intensity, IgG was added and the time-course of the fluorescence intensity was measured on a fluorometer connected with the optic fibers. Furthermore, the fluorescence response of a transient state was evaluated with the same system.When the Qdot-labeled protein A bound to IgG, fluorescence intensity decreased because of the inhibition by IgG. The degree of fluorescence decrease depends on the IgG concentration at a steady state and also in a transient state. (C) 2004 Elsevier B.V. All rights reserved.