The bloodless rat: a new model for macromolecular transport studies across lung endothelium.
The bloodless rat: a new model for macromolecular transport studies across lung endothelium.
复制标题
无血大鼠：跨肺内皮大分子转运研究的新模型。
DOI：
10.1152/ajpheart.1982.242.5.h890
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Neary,BA
中科院分区：
文献类型：
--
作者：
Schneeberger,EE;Neary,BA
To examine the effect of circulating proteins on the passage of intravenously injected native ferritin across pulmonary capillary endothelium, rats were exchange transfused with FC-43 fluorocarbon emulsion (FCE) under ether anesthesia. Protein concentration was reduced to less than 1.1 mg/ml by exchange transfusion, followed by FCE containing 15, 30, or 60 mg/ml of lyophilized rat serum protein (LRSP). Two minutes after ferritin injection lungs were prepared for ultrastructural morphometry. The diameter and numerical density of vesicles remained unchanged under all experimental conditions; however, at 0.6 mg/ml of circulating protein there was a 5- and 10-fold increase, respectively in percent vesicle (%VL) and basement membrane labeling (BML) by ferritin. This was reversible; at 60 mg/ml of circulating protein %VL and BML was indistinguishable from controls. Following a reduction of circulating protein to less than 1.1 mg/ml, the addition of 15 mg/ml LRSP reduced %VL but had no effect on BML. This suggests that in addition to shuttling vesicles there may be a second mechanism for the transport of ferritin, possibly involving transendothelial chains of vesicles.
