Differential responsiveness of prostatic acid phosphatase and prostate-specific antigen mRNA to androgen in prostate cancer cells
Differential responsiveness of prostatic acid phosphatase and prostate-specific antigen mRNA to androgen in prostate cancer cells
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DOI：
10.1006/cbir.2000.0433
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发表时间：
2000-01-01
影响因子：
3.9
通讯作者：
Lin, FF
中科院分区：
文献类型：
--
作者：
Lin, MF;Lee, MS;Lin, FF
雄激素调节人前列腺酸性磷酸酶（PAcP）和前列腺特异性抗原（PSA）这两种主要的前列腺上皮特异性分化抗原的表达。由于这两种酶作为前列腺上皮分化标志物的重要作用，我们研究了它们在LNCaP人前列腺癌细胞中在mRNA水平上的表达调控。有趣的是，培养基中的pH指示剂酚红促进了细胞生长。为了消除这种非特异性效应，使用了无酚红、类固醇减少的培养基。当高密度细胞在该培养基中生长时，5 α-二氢睾酮（DHT）抑制PAcP，但刺激PSA。然而，肿瘤促进剂佛波酯12-o-十四烷酰基佛波-13-乙酸酯（TPA）作为PAcP和PSA表达的有效抑制剂发挥作用。长期治疗与DHT以及TPA导致类似的下调蛋白激酶C和细胞PAcP的活动。因此，在LNCaP细胞中，PAcP和PSA mRNA的水平受到雄激素的差异调节。(C)北京大学出版社.
Androgens regulate the expression of both human prostatic acid phosphatase (PAcP) and prostate-specific antigen (PSA), two major prostate epithelium-specific differentiation antigens. Due to the important role of these two enzymes as prostate epithelium differentiation markers, we investigated their regulation of expression at the mRNA level in LNCaP human prostate carcinoma cells. Interestingly, phenol red, a pH indicator in the culture medium, promoted cell growth. To eliminate this non-specific effect, a phenol red-free, steroid-reduced medium was utilized. When high-density cells were grown in that medium, 5 alpha -dihydrotestosterone (DHT) suppressed PAcP but stimulated PSA. However, tumor promoter phorbol ester 12-o-tetradecanoyl phorbol-13-acetate (TPA) functioned as a potent inhibitor of both PAcP and PSA expression. Prolonged treatment with DHT as well as TPA resulted in a similar down-regulation of protein kinase C and cellular PAcP activities. Thus, the levels of PAcP and PSA mRNA are differentially regulated by androgens in LNCaP cells. (C) 2000 Academic Press.