Complementary iTRAQ Proteomic and Transcriptomic Analyses of Leaves in Tea Plant ( Camellia sinensis L.) with Different Maturity and Regulatory Network of Flavonoid Biosynthesis.
Complementary iTRAQ Proteomic and Transcriptomic Analyses of Leaves in Tea Plant ( Camellia sinensis L.) with Different Maturity and Regulatory Network of Flavonoid Biosynthesis.
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DOI：
10.1021/acs.jproteome.8b00578
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发表时间：
2018-11
影响因子：
4.4
通讯作者：
Liangyu Wu;Z. Fang;Jin-Ke Lin;Yun Sun;Zhi-Zheng Du;Yaling Guo;Jiangong Liu;Yuerong Liang;
中科院分区：
文献类型：
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作者：
Liangyu Wu;Z. Fang;Jin-Ke Lin;Yun Sun;Zhi-Zheng Du;Yaling Guo;Jiangong Liu;Yuerong Liang;
茶叶品质的好坏与采收时鲜叶的成熟度密切相关。本研究利用iTRAQ和RNA-SEQ技术研究了不同成熟度茶叶的蛋白质组和转录组分图谱。通过GO分类和KEGG注释的功能丰富分析，共鉴定出4455个蛋白质和27930个Uniges。测定了茶叶中儿茶素和黄酮醇类化合物的组成。总黄酮含量随着叶片成熟度的增加而降低，与莽草酸、苯丙素和类黄酮的蛋白质调控模式一致。ANR丰度与表儿茶素含量呈正相关，LAR丰度与儿茶素含量呈正相关(P<；0.05)。结合光合作用、初级代谢和转录因子讨论了类黄酮生物合成的生物合成网络。与成熟叶片相反，芽的光合作用和固碳活性最低，但类黄酮生物合成能力最高。SUS-INV开关可能是碳流转移到后续生化合成的重要节点。本研究综述了茶树不同生育期功能蛋白质组分的变化，并提出了初级代谢与类黄酮生物合成相关性的研究方向。
The quality of tea is highly related with the maturity of the fresh tea leaves at harvest. The present study investigated the proteomic and transcriptomic profiles of tea leaves with different maturity, using iTRAQ and RNA-seq technologies. A total of 4455 proteins and 27 930 unigenes were identified, with functional enrichment analyses of GO categorization and KEGG annotation. The compositions of flavonoids (catechins and flavonols) in tea leaves were determined. The total content of flavonoids decreased with leaf maturity, in accordance with the protein regulation patterns of shikimate, phenylpropanoid, and flavonoid pathways. The abundance of ANR had a positive correlation with epi-catechin content, while LAR abundance was positively related with catechin content ( P < 0.05). The biosynthetic network of flavonoid biosynthesis was discussed in combination with photosynthesis, primary metabolism, and transcription factors. Bud had the lowest activities of photosynthesis and carbon fixation but the highest flavonoid biosynthesis ability in opposite to mature leaf. SUS-INV switch might be an important joint for carbon flow shifting into the follow-up biochemical syntheses. This work provided a comprehensive overview on the functional protein profile changes of tea leaves at different growing stages and also proposed a research direction regarding the correlations between primary metabolism and flavonoid biosynthesis.