Changing patterns in frequency of recovery of five methicillin-resistant Staphylococcus aureus clones in Portuguese hospitals:: Surveillance over a 16-year period
Changing patterns in frequency of recovery of five methicillin-resistant Staphylococcus aureus clones in Portuguese hospitals:: Surveillance over a 16-year period
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DOI：
10.1128/jcm.00692-08
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发表时间：
2008-09-01
影响因子：
9.4
通讯作者：
de Lencastre, Herminia
中科院分区：
文献类型：
--
作者：
Aires-de-Sousa, Marta;Correia, Bruno;de Lencastre, Herminia
2006年6月至11月，从葡萄牙不同地区的11家医院共回收了629株非重复耐甲氧西林金黄色葡萄球菌MRSA分离株。所选菌株（n = 271，43%）进行了分型脉冲场凝胶电泳（PFGE），其中的代表还具有SPA分型，多位点序列分型，葡萄球菌盒式染色体mec（SCCmec）分型，并存在潘顿-瓦伦丁杀白细胞素（PVL）。将271株分离物分为13个不同的克隆型。三个大流行性克隆包括了大多数（i）EMRSA-15（54%）- PFGE A型，ST 22，spa t022，SCCmec IV -在所研究的11家医院中发现，并在其中7家医院中鉴定为主要克隆;（ii）纽约/日本克隆（17%）-- PFGE B、ST 5、spa型t067、SCCmec II --在9家医院中鉴定，并在4家医院中代表主要克隆;和（iii）巴西MRSA（17%）- PFGE C，ST 239，spa型t037，SCCmec IIIA -也在9家医院中检测到，但从未作为主要克隆。所有供试分离株均为PVL阴性。克隆EMRSA-15是目前在葡萄牙医院流通的主要MRSA克隆类型，但随着最近纽约/日本克隆的引入和传播，该国出现了新一波MRSA。20世纪90年代末在葡萄牙领先的巴西MRSA克隆正在下降，并逐渐被前两个克隆所取代。我们报告了葡萄牙第一个分离的SCCmec V型（ST 45）。
A total of 629 nonduplicate methicillin-resistant Staphylococcus aureus MRSA isolates were recovered between June and November 2006 from 11 hospitals located in different areas of Portugal. Selected isolates (n = 271, 43%) were typed by pulsed-field gel electrophoresis (PFGE), representatives of which were additionally characterized by spa typing, multilocus sequence typing, staphylococcal cassette chromosome mec (SCCmec) typing, and the presence of Panton-Valentine leukocidin (PVL). The 271 isolates were classified into 13 different clonal types. Three pandemic clones included the majority (n = 241, 88%) of the isolates and were observed in several hospitals: (i) EMRSA-15 (54%) - PFGE type A, ST22, spa type t022, SCCmec IV - was found in the 11 hospitals studied and was identified as the major clone in seven of them; (ii) the New York/Japan clone (17%) - PFGE B, ST5, spa type t067, SCCmec II - was identified in nine hospitals and represented the major clone in four; and (iii) the Brazilian MRSA (17%) - PFGE C, ST239, spa type t037, SCCmec IIIA - was also detected in nine hospitals but never as the main clone. All isolates tested were PVL negative. Clone EMRSA-15 is currently the predominant MRSA clonal type circulating in Portuguese hospitals, but a new wave of MRSA has emerged in the country with the recent introduction and spread of the New York/Japan clone. The Brazilian MRSA that was the leading clone in Portugal in the late 1990s is declining and being progressively replaced by the two former clones. We report the first isolate SCCmec type V (ST45) in Portugal.