A major host plant volatile, 1-octen-3-ol, contributes to mating in the legume pod borer, Maruca vitrata (Fabricius) (Lepidoptera: Crambidae)
A major host plant volatile, 1-octen-3-ol, contributes to mating in the legume pod borer, Maruca vitrata (Fabricius) (Lepidoptera: Crambidae)
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DOI：
10.1007/s00114-015-1297-0
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发表时间：
2015-10-01
影响因子：
1.8
通讯作者：
Torto, B.
中科院分区：
文献类型：
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作者：
Bendera, M.;Ekesi, S.;Torto, B.
豆科荚螟Maruca vitrata Fabricius（鳞翅目：豆蛾科）是豇豆的严重害虫，Maruca unguiculata （L.）Walp。（Fabales: Fabaceae），在撒哈拉以南非洲的研究主要集中在性信息素上，但寄主植物对性行为的作用尚未探讨。我们在实验室使用行为分析和化学分析来研究这种相互作用。我们发现，与对照相比，豇豆幼苗和叶片二氯甲烷提取物的存在分别使豆科豆荚螟的偶联性增加了33%和61%，这表明接触和嗅觉线索都参与其中。采用气相色谱-天线电联用（GC/EAD）和气相色谱-质谱联用（GC/MS）技术对豇豆叶提取物中的化合物进行鉴定。我们发现，昆虫的触角一致检测到四种成分，1-辛烯-3-醇是完整豇豆植株和叶片提取物释放的挥发物中共同和主要的成分。因此，我们研究了它在vitrata的偶联中的作用。在剂量反应试验中，与对照相比，随着化合物剂量的增加，1-辛烯-3-醇增加了vitrata的偶联性。我们的研究结果表明豇豆挥发性物质1-辛烯-3-醇参与了vitrata的性行为。
Previous studies on the legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae), a serious pest of cowpea, Vigna unguiculata (L.) Walp. (Fabales: Fabaceae), in sub-Saharan Africa have focused on sex pheromones, but the role of the host plant on sexual behavior has not been explored. We investigated this interaction in the laboratory using behavioral assays and chemical analyses. We found that the presence of cowpea seedlings and a dichloromethane extract of the leaf increased coupling in the legume pod borer by 33 and 61 %, respectively, compared to the control, suggesting the involvement of both contact and olfactory cues. We used coupled gas chromatography-electroantennographic detection (GC/EAD) and GC-mass spectrometry (GC/MS) to identify compounds from the cowpea leaf extract, detected by M. vitrata antenna. We found that the antennae of the insect consistently detected four components, with 1-octen-3-ol identified as a common and dominant component in both the volatiles released by the intact cowpea plant and leaf extract. We therefore investigated its role in the coupling of M. vitrata. In dose-response assays, 1-octen-3-ol increased coupling in M. vitrata with increasing dose of the compound compared to the control. Our results suggest that the cowpea volatile 1-octen-3-ol contributes to M. vitrata sexual behavior.