Histochemical detection of H2O2 by tissue printing as a precocious marker of rhizogenesis in grapevine
Histochemical detection of H2O2 by tissue printing as a precocious marker of rhizogenesis in grapevine
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DOI：
10.1016/s0981-9428(99)80019-9
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发表时间：
1998-11-01
影响因子：
6.5
通讯作者：
Amâncio, S
中科院分区：
文献类型：
--
作者：
Neves, C;Sá, MC;Amâncio, S
对于组织学检测和定位的H2 O2作为一个早熟的标志物的生根，一个敏感的组织打印法在新鲜切割的茎切片。利用该技术可以对葡萄属葡萄不定根发生过程中组织中的H_2O_2进行检测和定位。插条，用生长素诱导。在未诱导的茎中，H_2O_2优先与表皮、髓薄壁组织和维管形成层、木质部和韧皮部结合，表现为缺乏H_2O_2。组织打印生长素处理的插条允许识别一组典型的特征，允许定义9个组织学模式在根诱导和伸长。在生长素处理的第二天，维管形成层变得不规则，髓射线失去了以前的组织;在第四天，维管形成层是完全不清楚的。生长素去除后，它是可以确定的分生组织最初作为无色的斑点，然后不定根原基;维管形成层开始重新组织，除了在根原基的区域。随着根的伸长，可以将根维管系统识别为有色细胞，而皮层细胞为无色细胞，与干模式形成对比。不含生长素的对照材料允许确认所观察到的模式与生根密切相关。(C)爱思唯尔，巴黎。
For the histological detection and localisation of H2O2 as a precocious marker of rhizogenesis, a sensitive tissue-printing assay in freshly cut stem sections was used. With this technique, we could detect and localise H2O2 present in tissues during the adventitious rooting process of Vitis vinifera L. cuttings, induced in vitro by auxin. In non induced stems, H2O2 is preferentially associated to epidermis, pith parenchyma and vascular cambium, xylem and phloem appearing devoid of H2O2. Tissue-printing of auxin treated cuttings permitted the identification of a typical set of characteristics that allowed to define nine histological patterns during root induction and elongation. On the second day of auxin treatment, vascular cambium became irregular, with medullary rays losing their previous organisation; on day four, the vascular cambium was totally indistinct. After auxin removal, it was possible to identify the meristemoids initially as uncoloured spots and afterwards the adventitious root primordia; vascular cambium began to re-organise except in the zones of the root primordia. As root elongated, it was possible to identify the root vascular system as coloured cells, and the cortex cells as non coloured ones, contrasting with the stem pattern. Control material without auxin allowed the confirmation that the observed patterns were well associated to rhizogenesis. (C) Elsevier, Paris.