The Effect of Doxycycline Hyclate, Chlorhexidine Gluconate, and Minocycline Hydrochloride on Osteoblastic Proliferation and Differentiation In Vitro
The Effect of Doxycycline Hyclate, Chlorhexidine Gluconate, and Minocycline Hydrochloride on Osteoblastic Proliferation and Differentiation In Vitro
复制标题
DOI：
10.1902/jop.2009.080574
复制
发表时间：
2009-06-01
影响因子：
4.3
通讯作者：
Ciancio, Sebastian G.
中科院分区：
文献类型：
--
作者：
Almazin, Salah M.;Dziak, Rosemary;Ciancio, Sebastian G.
背景：本研究的目的是确定三种局部递送系统的活性物质，水合多西环素10% （DOXY）、葡萄糖酸氯己定2.5 mg （CHX）和盐酸米诺环素I mg （MINO）对成骨细胞增殖和分化的影响。方法：采用对照成骨细胞（OB）、OB + DOXY、OB + CHX、OB + MINO四组。台盼蓝法和MTT[3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑]法检测成骨细胞活力。通过测定碱性磷酸酶水平检测细胞分化。光镜和扫描电镜观察成骨细胞形态。结果：在0.5 mg/ml浓度下，台盼蓝试验显示，与对照组相比，DOXY、MINO和CHX对成骨细胞有明显的毒性作用，平均细胞活力分别为84%、74%和51% (P
Background: The purpose of this study was to determine the effect of the active substance of three types of local delivery systems, doxycycline hyclate 10% (DOXY), chlorhexidine gluconate, 2.5 mg (CHX), and minocycline hydrochloride, I mg (MINO), on osteoblastic cell proliferation and differentiation.Methods: There were four groups: control osteoblastic cells (OB) alone, OB + DOXY, OB + CHX, and OB + MINO. Trypan blue and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays were used to test osteoblastic cell viability. Cell differentiation was tested by measuring alkaline phosphatase levels. Osteoblast morphology was investigated by light and scanning electron microscopy.Results: At a concentration of 0.5 mg/ml, the Trypan blue test showed that DOXY, MINO, and CHX had significant toxicity effects on osteoblast cells compared to the control group, with a mean cell viability of 84%, 74%, and 51%, respectively (P