Prognostic value of uPA and p53 accumulation measured by quantitative biochemical assays in 1245 primary breast cancer patients: a multicentre study.
Prognostic value of uPA and p53 accumulation measured by quantitative biochemical assays in 1245 primary breast cancer patients: a multicentre study.
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DOI：
10.1038/sj.bjc.6690389
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发表时间：
1999-05
影响因子：
8.8
通讯作者：
Asselain, B
中科院分区：
文献类型：
--
作者：
Broët, P;Spyratos, F;Romain, S;Quillien, V;Daver, A;Ricolleau, G;Rallet, A;Toulas, C;Asselain, B
本回顾性多中心研究的目的是评估尿激酶纤溶酶原激活物（uPA）和p53水平在一个大系列的原发性乳腺癌的预后价值，使用自动定量发光法。从7个法国机构收集了1245例可手术乳腺肿瘤样本，并对患者进行了中位75个月的随访。通过免疫发光技术（LIA）测定的细胞质中uPA和p53水平中位数分别为0.31和0.20 ng mg−1蛋白。在单变量分析中，高水平的uPA和p53与较短的疾病特异性生存期、无病间期和远处无复发间期相关。uPA值低于第20百分位数的患者的5年生存率为95.5%，高于第80百分位数的患者的5年生存率为77.5%。p53值低于第20百分位数的患者的5年生存率为91.0%，高于第80百分位数的患者的5年生存率为77.6%。在多变量分析中，校正肿瘤大小、组织学分级、淋巴结受累和雌激素受体状态后，疾病相关死亡的风险随uPA水平升高而增加。高水平的uPA也与较短的无病间期和较远的无复发间期相关。在淋巴结阴性患者中，高水平的uPA仍然与三种结局密切相关。当调整其他预后因素后，p53不再与预后显著相关。由于其快速和简单的应用程序，以常规制备的胞质溶胶，这种LIA可能是有用的评估uPA在原发性乳腺癌的预后影响，特别是在淋巴结阴性患者。根据我们的研究结果，当uPA被纳入多变量分析时，p53积累的预后价值是有限的。© 1999癌症研究运动
The purpose of this retrospective multicentre study was to assess the prognostic value of urokinase plasminogen activator (uPA) and p53 levels in a large series of primary breast cancer, using an automatic quantitative luminometric method. Samples of 1245 operable breast tumours were collected from seven French institutions and patients were followed for a median of 75 months. The median uPA and p53 levels assayed in cytosols by means of the immunoluminometric technique (LIA) were 0.31 and 0.20 ng mg−1 of protein respectively. In univariate analysis, high levels of uPA and p53 were associated with shorter disease-specific survival, disease-free interval, and distant recurrence-free interval. The 5-year survival rates were 95.5% among patients with uPA values below the 20th percentile, and 77.5% in those with values above the 80th percentile. The 5-year survival rates were 91.0% in patients with p53 values below the 20th percentile, and 77.6% in those with values above the 80th percentile. In multivariate analysis, the risk of disease-related death increased with uPA levels after adjustment for tumour size, histological grade, lymph node involvement, and estrogen receptor status. A high level of uPA was also related to a shorter disease-free interval and distant recurrence-free interval. In node-negative patients, a high level of uPA remained strongly related to the three outcomes. When adjusted for other prognostic factors, p53 was no longer significantly related to the outcomes. Given its rapidity and simple application to routinely prepared cytosols, this LIA may be useful for evaluating the prognostic impact of uPA in primary breast cancer, particularly in node-negative patients. According to our results, the prognostic value of p53 accumulation is limited when uPA is included in multivariate analysis. © 1999 Cancer Research Campaign
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影响因子：
8.8
作者：
BORG, A;LENNERSTRAND, J;BRUNDELL, J
通讯作者：
BRUNDELL, J
影响因子：
8.4
作者：
Ferno, M;Bendahl, PO;Killander, D
通讯作者：
Killander, D
DOI：
10.1093/jnci/85.3.200
发表时间：
1993-02-03
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
ALLRED, DC;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
8.8
作者：
Bouchet, C;Spyratos, F;Martin, P M;Hacene, K;Gentile, A;Oglobine, J
通讯作者：
Oglobine, J