Cell damage resulting from the labeling of rat lymphocytes and HeLa S3 cells with In-111 oxine.
Cell damage resulting from the labeling of rat lymphocytes and HeLa S3 cells with In-111 oxine.
复制标题
In-111 oxine 标记大鼠淋巴细胞和 HeLa S3 细胞造成的细胞损伤。
作者：
P. M. Chisholm;H. Danpure;G. Healey;S. Osman
用不同剂量的铟-111对大鼠胸导管淋巴细胞和HeLa S3细胞进行体外标记。测试标记的大鼠淋巴细胞在同基因大鼠体内正常再循环的能力；标记的HeLa S3细胞在组织培养中分裂形成集落的能力。根据这些标准，这两种类型的细胞在用少量铟-111恶辛标记时表现正常，但在较高剂量时明显受损。对于HeLa S3细胞，有证据表明这种损伤主要是由辐射引起的。这些发现可能会限制In-111 oxine作为临床细胞标记的使用。
Rat thoracic-duct lymphocytes and HeLa S3 cells were labeled in vitro with different amounts of indium-111 oxine. The labeled rat lymphocytes were tested for their ability to recirculate normally in syngeneic rats; the labeled HeLa S3 cells for their ability to divide to form colonies in tissue culture. Both cell types behaved normally by these criteria when labeled with small amounts of indium-111 oxine but at higher doses were obviously damaged. Evidence was obtained for the HeLa S3 cells that this damage was primarily radiation-induced. These findings may impose limitations on the use of In-111 oxine as a cell label for clinical purposes.