A correlation between knee cartilage degradation observed by arthroscopy and synovial proteinases activities
A correlation between knee cartilage degradation observed by arthroscopy and synovial proteinases activities
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DOI：
10.1016/s0009-9120(03)00029-8
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发表时间：
2003-06-01
影响因子：
2.8
通讯作者：
Coletta, M
中科院分区：
文献类型：
--
作者：
Marini, S;Fasciglione, GF;Coletta, M
目的：已经进行了一项新的研究来表征金属蛋白酶（即，MMP-1、MMP-2、MMP-3、MMP-8、MMP-9和MMP-13）及其抑制剂（即，TIMP-1和TIMP-2）在滑液中的表达，这些患者（n = 56）具有不同程度的软骨损伤或膝关节炎，通过关节镜检查确定和分类。设计和方法：酶谱法、蛋白质印迹法和ELISA试验已被用于将关节镜检查确定的疾病阶段与不同MMP及其抑制剂的量和激活状态相关联。所得数据的分析表明，关节镜观察到的软骨降解程度与一些滑液MMPs（特别是MMP-2和MMP-13）的活性以及TIMP-2对MMP-2的抑制作用的降低密切相关;此外，重约125 kDa的丝氨酸蛋白酶仅出现在严重软骨降解的患者中，即，膝关节炎。结论：总的来说，这是第一项研究，其中分析了滑膜MMPs/其他蛋白酶活性和TIMPs与不同程度骨关节炎患者的关节镜检查结果严格相关。结果表明，特定的MMP活性和TIMPs的量及其抑制效率之间的不平衡是疾病演变的关键，并且与疾病阶段有关。(C)2003年加拿大临床化学家协会。All rights reserved.
Objective: A novel study has been carried out to characterize the amount and activity levels of metalloproteinases (i.e., MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-13) and of their inhibitors (i.e., TIMP-1 and TIMP-2) in synovial fluid from patients (n = 56) with different degrees of either chondral lesions or knee arthritis identified and classified by arthroscopy.Design and methods: Zymographies, Western blotting and ELISA tests have been used to correlate the disease stage, as determined by arthroscopy, and both the amount and the activation state of different MMPs and of their inhibitors.Results: Analysis of data obtained demonstrates that the degree of cartilage degradation, as seen by arthroscopy, is strictly related to the activity of some synovial MMPs, in particular MMP-2 and MMP-13 and on reduced inhibitory effect of MMP-2 by TIMP-2; in addition, a serine protease weighing about 125 kDa appears only in patients with severe cartilage degradation, i.e., with knee arthritis.Conclusions: On the whole, this is the first study in which an analysis of synovial MMPs/other proteinases activity and TIMPs has been strictly related to arthroscopy results in patients with different degrees of osteoarthritis. Results indicate that an imbalance between specific MMP activities and the amount of TIMPs and of its inhibitory efficiency is crucial for the disease evolution and it is related to the disease stage. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.