Evolutionary expression expression differences of creatine synthesis-related genes: Implications for skeletal muscle metabolism in fish
Evolutionary expression expression differences of creatine synthesis-related genes: Implications for skeletal muscle metabolism in fish
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DOI：
10.1038/s41598-019-41907-6
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发表时间：
2019-04-01
影响因子：
4.6
通讯作者：
Goldammer, Tom
中科院分区：
文献类型：
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作者：
Borchel, Andreas;Verleih, Marieke;Goldammer, Tom
肌酸/磷酸肌酸系统是哺乳动物的主要能量缓冲，但在鱼类中几乎没有记录。我们测定了该系统的主要酶甘氨酸脒基转移酶（GATM）、胍基乙酸N-甲基转移酶（GAMT）和肌型肌酸激酶（CKM）在鱼类和哺乳动物的肾脏、肝脏和肌肉组织中的基因表达。CKM强烈表达在所有检查的物种的肌肉。相反，GATM和GAMT在鱼类肌肉组织中强烈表达，但在哺乳动物中没有。这表明肌酸的合成和使用在哺乳动物中是空间分离的，但在鱼类中不是，这得到了25个物种的RNA-Seq数据的支持。鱼类肌肉中氨基酸代谢的差异沿着甲硫氨酸腺苷转移酶基因的表达，而哺乳动物肌肉中的差异进一步支持了鱼类肌肉的中心代谢作用，因此支持了高等和低等脊椎动物中肌酸/磷酸肌酸生物合成系统的不同组织。
The creatine/phosphocreatine system is the principal energy buffer in mammals, but is scarcely documented in fish. We measured the gene expression of major enzymes of this system, glycine amidinotransferase (GATM), guanidinoacetate N-methyltransferase (GAMT) and muscle-type creatine kinase (CKM) in kidney, liver, and muscle tissues offish and mammals. CKM was expressed strongly in the muscles of all examined species. In contrast, GATM and GAMT were strongly expressed in the muscle tissue offish, but not of mammals. This indicates that creatine synthesis and usage are spatially separated in mammals, but not in fish, which is supported by RNA-Seq data of 25 species. Differences in amino acid metabolism along with methionine adenosyltransferase gene expression in muscle from fishes but not mammals further support a central metabolic role of muscle in fish, and hence different organization of the creatine/phosphocreatine biosynthesis system in higher and lower vertebrates.