STEPWISE PROGRESSION OF B-LINEAGE DIFFERENTIATION SUPPORTED BY INTERLEUKIN-7 AND OTHER STROMAL CELL MOLECULES
STEPWISE PROGRESSION OF B-LINEAGE DIFFERENTIATION SUPPORTED BY INTERLEUKIN-7 AND OTHER STROMAL CELL MOLECULES
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DOI：
10.1084/jem.171.5.1683
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发表时间：
1990-05-01
影响因子：
15.3
通讯作者：
NISHIKAWA, SI
中科院分区：
文献类型：
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作者：
HAYASHI, SI;KUNISADA, T;NISHIKAWA, SI
以il -7和il -7缺陷基质细胞系PA6为分离生长信号，研究早期B前体细胞的体外生长情况。B细胞的发育经历了三个不同于生长信号需求的连续阶段。第一阶段的细胞仅需要PA6进行增殖，分化到第二阶段，第二阶段的细胞生长需要PA6和IL-7。当第二阶段的细胞有IL-7可用时，它们在PA6层上广泛增殖，并且一些细胞仅在IL-7的作用下获得增殖能力。然而，这种生长信号需求的顺序变化并不是沿着时间计划自主进行的。考虑到它主要是由Ig基因重排的结果指导的可能性。这种生长控制模式可以解释为什么在B谱系分化过程中，在容易出错的Ig基因重排过程中，只有功能正常的B细胞被选择。
Growth of early B precursor cells was investigated in vitro by using rIL-7 and IL-7-defective stromal cell line PA6 as separate growth signals. B cell development proceeds through three sequential stages different from the growth signal requirement. The cells in the first stage require PA6 alone for the proliferation, and differentiate into the second stage, which requires both PA6 and IL-7 for its growth. When IL-7 is available for the cells in the second stage, they proliferate extensively on the PA6 layer, and some acquire the ability to proliferate in response to IL-7 alone. This sequential change of growth signal requirement, however, does not proceed autonomously along the time schedule. The possibility that it is primarily directed by the result of Ig gene rearrangement is considered. This mode of growth control may explain why only functional B cells are selected in the error-prone process of Ig gene rearrangement during B lineage differentiation.