Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei
Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei
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DOI：
10.1016/j.aquaculture.2009.02.004
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发表时间：
2009-05
期刊：
影响因子：
4.5
通讯作者：
L. Phuoc;M. Corteel;N. C. Thanh;H. Nauwynck;M. Pensaert;V. Alday‐Sanz;W. Broeck;P. Sorgeloos;P. Bossier
中科院分区：
文献类型：
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作者：
L. Phuoc;M. Corteel;N. C. Thanh;H. Nauwynck;M. Pensaert;V. Alday‐Sanz;W. Broeck;P. Sorgeloos;P. Bossier
本研究旨在探讨弧菌的剂量和攻毒途径对细菌生长的影响。对无特定病原体（SPF）南美白对虾（Penaeus vannamei）与白色斑点综合征病毒（WSSV）共感染的影响。幼虾首先注射WSSV，剂量为30 SID 50虾-1（SID 50= 50%终点的虾感染剂量），24 h后注射10 3，10 4，10 5或10 6 CFU虾-1的V. campbellii。对照组在实验过程中没有死亡，除了接受10 6 CFU虾-1的对照组（35-65%）。在WSSV接种的对虾中，在注射后144-360 h（hpi）达到100%的累积死亡率。当注射至少10 4CFU的V. campbellii时，WSSV感染的虾死亡更快，在48-96 hpi时达到100%的累积死亡率。结果表明，注射康氏弧菌后6 h，共感染的濒死对虾血淋巴中康氏弧菌的密度显著高于单独注射康氏弧菌的对虾。有没有WSSV感染细胞的数量之间的差异WSSV单独接种的虾，相比，双重接种的。首先注射WSSV，24 h（或48 h）后通过浸泡暴露于10 6、10 7或10 8 CFU ml− 1的V. campbellii的虾没有显示出任何加速死亡。当WSSV感染的虾用另一种弧菌（Vibrio harveyi BB 120）攻击时，在注射10 6 CFU虾-1的V. harveyi BB 120的WSSV感染的虾中没有观察到加速死亡。
This study was conducted to investigate the effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Penaeus vannamei shrimp. Juvenile shrimp were first injected with WSSV at a dose of 30 SID 50 shrimp− 1 (SID 50= shrimp infectious dose with 50% endpoint) and 24 h later with 10 3, 10 4, 10 5 or 10 6 CFU shrimp− 1 of V. campbellii. Controls did not die during the experiment, except the ones that received 10 6 CFU shrimp− 1 (35–65%). In WSSV-inoculated shrimp, the 100% cumulative mortality were reached at 144–360 h post injection (hpi). WSSV-infected shrimp died much faster when injected with at least 10 4 CFU of V. campbellii with the 100% cumulative mortality reached at 48–96 hpi of virus. The density of V. campbellii in haemolymph of co-infected moribund shrimp collected 6 h after V. campbellii injection was significantly higher than that in shrimp injected with V. campbellii only. There was no difference in the number of WSSV-infected cells between shrimp inoculated with WSSV only, compared to dually inoculated ones. Shrimp which were first injected with WSSV and 24 h (or 48 h) later exposed to 10 6, 10 7, or 10 8 CFU ml− 1 of V. campbellii by immersion did not show any accelerated mortality. When WSSV-infected shrimp were challenged with another Vibrio species, V. harveyi BB120, no accelerated mortality was noted in WSSV-infected shrimp injected with 10 6 CFU shrimp− 1 of V. harveyi BB120.