Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas.
Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas.
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发表时间：
1981-05
期刊：
影响因子：
11.2
通讯作者：
J. Rheinwald;M. Beckett
中科院分区：
文献类型：
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作者：
J. Rheinwald;M. Beckett
我们已经建立了细胞系，从六个人鳞状细胞癌（SCC）的表皮和舌，使用培养方法以前开发的克隆生长和正常角质形成细胞的连续培养。SCC细胞系在裸鼠中均形成快速生长、分化良好的SCC或进行性生长的鳞状囊肿。与正常角质形成细胞相反，SCC细胞形成未分层或非常差的分层集落，并且不需要表皮生长因子来持续生长。与正常角质形成细胞一样，SCC细胞系对成纤维细胞饲养层的需求不同，以支持克隆生长。只有一个系在半固体培养基中以高效率形成大的、逐渐生长的集落;其他五个系在半固体培养基中仅表现出少量的败育生长，之后细胞似乎迅速退化。这些结果表明，鳞状细胞癌通常在培养物中生长为已建立的细胞系，但它们通常具有与正常角质形成细胞相似的体外生长要求。因此，无论是半固体培养基还是标准表面培养基都不适合于起始原代SCC培养或从致癌物处理的角质形成细胞群体中选择转化体，因为它们不提供允许许多恶性角质形成细胞生长的条件。
We have established cell lines from six human squamous cell carcinomas (SCC) of the epidermis and tongue, using culture methods previously developed for clonal growth and serial cultivation of normal keratinocytes. The SCC lines all form rapidly growing, well-differentiated SCC's or progressively growing squamous cysts in nude mice. In contrast to normal keratinocytes, SCC cells form unstratified or very poorly stratifying colonies and do not require epidermal growth factor for sustained growth. The SCC lines vary in their requirement for a fibroblast feeder layer to support clonal growth, as normal keratinocytes possess. Only one line forms large, progressively growing colonies at high efficiency in semisolid medium; the other five lines exhibit only a small amount of abortive growth in semisolid medium, after which the cells appear to rapidly degenerate. These results demonstrate that SCC's often grow as established lines in culture, but they frequently possess in vitro growth requirements similar to those of normal keratinocytes. Consequently, neither semisolid medium nor standard surface culture media are appropriate for initiating primary SCC cultures or for selecting transformants out of carcinogen-treated keratinocyte populations, because they do not provide conditions permissive for the growth of many malignant keratinocytes.