Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors.
Spontaneous Formation of RNA Strands, Peptidyl RNA, and Cofactors.
复制标题
DOI：
10.1002/anie.201506593
复制
发表时间：
2015-11-23
期刊：
影响因子：
--
通讯作者：
Richert C
中科院分区：
文献类型：
--
作者：
Jauker M;Griesser H;Richert C
生物化学机制是如何从简单的前体进化而来的，这是一个悬而未决的问题。在这里，我们表明，核糖核苷酸和氨基酸缩合肽基RNA的酶的情况下，建立遗传复制的条件下。可以编码遗传信息的RNA链的非模板形成、连接到RNA的肽基链的形成以及辅因子NAD+、FAD和ATP的形成都在相同的条件下发生。在肽基RNA中，肽链是与核糖核苷酸氨基磷酸酯连接的。当提供模拟膜内部的亲脂相时，从初始库中选择具有长肽链的肽基RNA，并且在酸化时释放游离肽。我们的研究结果表明，遗传、催化和代谢的关键分子可以在相同的条件下出现，没有矿物表面，没有酶，也不需要化学预活化。
How the biochemical machinery evolved from simple precursors is an open question. Here we show that ribonucleotides and amino acids condense to peptidyl RNAs in the absence of enzymes under conditions established for genetic copying. Untemplated formation of RNA strands that can encode genetic information, formation of peptidyl chains linked to RNA, and formation of the cofactors NAD+, FAD, and ATP all occur under the same conditions. In the peptidyl RNAs, the peptide chains are phosphoramidate-linked to a ribonucleotide. Peptidyl RNAs with long peptide chains were selected from an initial pool when a lipophilic phase simulating the interior of membranes was offered, and free peptides were released upon acidification. Our results show that key molecules of genetics, catalysis, and metabolism can emerge under the same conditions, without a mineral surface, without an enzyme, and without the need for chemical pre-activation.