Revisiting the intrinsic mycobiome in pancreatic cancer.
Revisiting the intrinsic mycobiome in pancreatic cancer.
复制标题
重新审视胰腺癌的内在真菌组。
DOI：
10.1038/s41586-023-06292-1
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发表时间：
2023
期刊：
影响因子：
64.8
通讯作者：
Allen,PeterJ
中科院分区：
文献类型：
--
作者：
Fletcher,AshleyA;Kelly,MatthewS;Eckhoff,AustinM;Allen,PeterJ
越来越多的文献表明，人类微生物组的改变是疾病发生和进展的原因。Aykut等人1提供的证据表明，肠道真菌微生物组（“真菌组”）的改变以及胰腺组织中存在的真菌成分（特别是马拉色菌属的真菌成分）沿着与胰腺肿瘤发生相关。在分析原始文章中提供的人类测序数据时，我们在胰腺组织样本中发现了很少的真菌读数，并且没有发现健康参与者和胰腺导管腺癌（PDAC）患者之间胰腺或肠道真菌群落组成的差异。我们对这些数据的重新分析不支持内在胰腺真菌组与人类PDAC的发展之间的关联，并说明了分析来自低生物量样品的微生物组测序数据的挑战。Aykut等人使用真菌的内部转录间隔区（ITS）区域的序列分析，表征了5个健康人类胰腺组织样品的真菌组，13份PDAC组织样本和18份PDAC患者粪便样本。他们还对来自健康参与者的9个粪便样本进行了测序，尽管这些数据没有在论文中列出。仅关注这些人类样本的分析，我们检索了通过国家生物技术信息中心（NCBI）序列读取档案（SRA）公开的原始测序数据，以确认论文中的发现，并比较健康参与者和PDAC患者的肠道和胰腺真菌组。
A growing body of literature suggests that alterations in the human microbiome are causative of disease initiation and progression. Aykut et al. 1 have provided evidence that alterations in the gut fungal microbiome (the ‘mycobiome’), along with the presence of fungal elements within pancreatic tissue (specifically those of the genus Malassezia), are associated with pancreatic oncogenesis. On analysing the human sequencing data presented in the original article, we found few fungal reads in pancreatic tissue samples and did not identify differences in pancreatic or gut mycobiome composition between healthy participants and patients with pancreatic ductal adenocarcinoma (PDAC). Our reanalysis of these data does not support an association between an intrinsic pancreatic mycobiome and the development of human PDAC, and illustrates the challenges in analysing microbiome sequencing data from low-biomass samples.Using sequence analysis of the internal transcribed spacer (ITS) region of fungi, Aykut et al. characterized the mycobiome of 5 healthy human pancreatic tissue samples, 13 PDAC tissue samples and 18 faecal samples from patients with PDAC. They also sequenced nine faecal samples from healthy participants, although these data were not presented in the paper. Focusing only on the analysis of these human samples, we retrieved the raw sequencing data that were publicly available through the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) to confirm the findings from the paper and to compare the gut and pancreatic mycobiomes of the healthy participants and patients with PDAC.