Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix
Distribution of interstitial cells of Cajal and nitrergic neurons in normal and diabetic human appendix
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DOI：
10.1111/j.1365-2982.2007.01040.x
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发表时间：
2008-04-01
影响因子：
3.5
通讯作者：
Farrugia, G.
中科院分区：
文献类型：
--
作者：
Miller, S. M.;Narasimhan, R. A.;Farrugia, G.
本研究的目的是确定肠神经和Cajal间质细胞（ICC）在正常人类阑尾和1型糖尿病患者中的分布。从1型糖尿病患者和非糖尿病对照组中收集阑尾。采用三维重建法测定神经体积和ICC，计数神经元一氧化氮合酶（nNOS）表达量。肠神经节分布于肌丛区和纵肌内。ICC遍布肌肉层。在糖尿病中，c-Kit阳性的ICC体积显著减少，表达nNOS的神经元也显著减少。总之，我们描述了ICC和肠神经在健康和糖尿病中的分布。数据还表明，人类阑尾，一个现成的人体组织来源，可能是研究运动障碍的有用模型。
The objective of this study was to determine the distribution of enteric nerves and interstitial cells of Cajal (ICC) in the normal human appendix and in type 1 diabetes. Appendixes were collected from patients with type 1 diabetes and from non-diabetic controls. Volumes of nerves and ICC were determined using 3-D reconstruction and neuronal nitric oxide synthase (nNOS) expressing neurons were counted. Enteric ganglia were found in the myenteric plexus region and within the longitudinal muscle. ICC were found throughout the muscle layers. In diabetes, c-Kit positive ICC volumes were significantly reduced as were nNOS expressing neurons. In conclusion, we describe the distribution of ICC and enteric nerves in health and in diabetes. The data also suggest that the human appendix, a readily available source of human tissue, may be useful model for the study of motility disorders.