Hepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation.
Hepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation.
复制标题
肝细胞移植：用于肝细胞移植的细胞表技术。
DOI：
10.1007/s40472-017-0156-7
复制
发表时间：
2017
影响因子：
2.1
通讯作者：
Okano T
中科院分区：
文献类型：
--
作者：
Tatsumi K;Okano T
我们将综述细胞片层技术作为一种可行的组织工程方法的最新进展。具体而言，我们将重点关注使用细胞片技术工程化功能性肝脏组织的技术进步，以及细胞片对肝脏疾病的相关治疗效果，重点关注血友病。使用肝细胞的基于细胞的疗法最近已被探索为具有多种形式的肝病患者的新治疗模式。我们已经开发了一种细胞片技术，该技术允许以单片层的形式收获细胞。我们已经成功地在小鼠中通过堆叠由原代肝细胞组成的细胞片来构建功能性肝组织。作为血友病的治疗措施，我们还成功地通过移植由基因修饰的自体细胞组成的细胞片来治疗血友病小鼠。使用细胞片层技术的组织工程为患有各种类型肝病的患者提供了创造新的治疗选择的机会。
We will review the recent developments of cell sheet technology as a feasible tissue engineering approach. Specifically, we will focus on the technological advancement for engineering functional liver tissue using cell sheet technology, and the associated therapeutic effect of cell sheets for liver diseases, highlighting hemophilia. Cell-based therapies using hepatocytes have recently been explored as a new therapeutic modality for patients with many forms of liver disease. We have developed a cell sheet technology, which allows cells to be harvested in a monolithic layer format. We have succeeded in fabricating functional liver tissues in mice by stacking the cell sheets composed of primary hepatocytes. As a curative measure for hemophilia, we have also succeeded in treating hemophilia mice by transplanting of cells sheets composed of genetically modified autologous cells. Tissue engineering using cell sheet technology provides the opportunity to create new therapeutic options for patients with various types of liver diseases.