Courtship interactions stimulate rapid changes in GnRH synthesis in male ring doves.
Courtship interactions stimulate rapid changes in GnRH synthesis in male ring doves.
复制标题
DOI：
10.1016/j.yhbeh.2008.07.005
复制
发表时间：
2008-11
影响因子：
3.5
通讯作者：
Buntin JD
中科院分区：
文献类型：
--
作者：
Mantei KE;Ramakrishnan S;Sharp PJ;Buntin JD
许多鸟类和哺乳动物的下丘脑-垂体-性腺 (HPG) 轴会因繁殖伙伴之间的社交或性互动而发生变化。虽然 GnRH 神经元活动的改变在刺激这些变化中发挥着重要作用，但尚不清楚急性行为诱导的 GnRH 释放改变是否伴随着 GnRH 合成的平行变化。为了研究这种关系，我们检查了雄性环斑鸠与雌性环斑鸠短暂求偶互动后大脑中 GnRH 神经元活性的变化。先前的研究表明，这种相互作用会增加雄性鸽子在与雌性交配后 24 小时求爱时的血浆 LH，但不会增加 1 小时内的血浆 LH。在第一项研究中，与保持与雌性隔离的对照雄性相比，允许向雌性求爱 2 小时的雄性下丘脑视前区 (POA) 中显示 GnRH-I 免疫细胞化学标记的细胞多 60%。为了确定 POA 中 GnRH 基因表达的增加是否先于 GnRH 免疫反应性的增加，在与雌性求偶相互作用 1 小时后，通过原位杂交测量 POA 中可检测到的 GnRH-I mRNA 的细胞数量的变化。在第二项研究中，求爱的雄性在该区域表现出比孤立的对照雄性多 40% 的 GnRH-I 细胞。另外两个间脑区域的 GnRH 免疫反应神经元未能表现出这些求爱引起的变化。求偶 1 或 2 小时后血浆 LH 没有升高。这些结果表明，POA 中 GnRH-I 的释放（可能负责求偶诱导的垂体和性腺激活）伴随着 GnRH 合成的快速增加，该合成发生在血浆 LH 水平增加之前。我们认为 GnRH 合成的增加对于支持 HPG 轴激活的延长是必要的，这种激活在该物种的求偶和筑巢活动的 5-10 天期间可见。
Many birds and mammals show changes in the hypothalamo-pituitary-gonadal (HPG) axis in response to social or sexual interactions between breeding partners. While alterations in GnRH neuronal activity play an important role in stimulating these changes, it remains unclear if acute behaviorally-induced alterations in GnRH release are accompanied by parallel changes in GnRH synthesis. To investigate this relationship, we examined changes in the activity of GnRH neurons in the brains of male ring doves following brief periods of courtship interactions with females. Such interactions have been previously shown to increase plasma LH in courting male doves at 24 h, but not at 1 h, after pairing with females. In the first study, males allowed to court females for 2 h had 60% more cells that showed immunocytochemical labeling for GnRH-I in the preoptic area (POA) of the hypothalamus than did control males that remained isolated from females. To determine whether an increase in GnRH gene expression preceded this increase in GnRH immunoreactivity in the POA, changes in the number of cells with detectable GnRH-I mRNA in the POA were measured by in situ hybridization following a 1 h period of courtship interactions with females. In this second study, courting males exhibited 40% more cells with GnRH-I in this region than did isolated control males. GnRH-immunoreactive neurons in two other diencephalic regions failed to show these courtship-induced changes. Plasma LH was not elevated after 1 or 2 h of courtship. These results demonstrate that the release of GnRH-I in the POA that is presumably responsible for courtship-induced pituitary and gonadal activation is accompanied by a rapid increase in GnRH synthesis that occurs before plasma LH levels increase. We suggest that this increase in GnRH synthesis is necessary to support the extended period of HPG axis activation that is seen in this species during the 5–10 day period of courtship and nest building activity.
登录
查看更多内容
影响因子：
3.2
作者：
Gore, AC;Wersinger, SR;Rissman, EF
通讯作者：
Rissman, EF
影响因子：
3.5
作者：
CHENG, MF;FOLLETT, BK
通讯作者：
FOLLETT, BK
影响因子：
3.5
作者：
DUNN, IC;CHEN, Y;SANG, HM
通讯作者：
SANG, HM
影响因子：
3.6
作者：
FEDER, HH;STOREY, A;SILVER, R
通讯作者：
SILVER, R
影响因子：
2.7
作者：
HAHN, TP;BALL, GF
通讯作者：
BALL, GF