INVOLVEMENT OF A POSSIBLE CHAPERONIN IN THE EFFICIENT EXPRESSION OF A CLONED CRYIIA DELTA-ENDOTOXIN GENE IN BACILLUS-THURINGIENSIS
INVOLVEMENT OF A POSSIBLE CHAPERONIN IN THE EFFICIENT EXPRESSION OF A CLONED CRYIIA DELTA-ENDOTOXIN GENE IN BACILLUS-THURINGIENSIS
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DOI：
10.1111/j.1365-2958.1992.tb00874.x
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发表时间：
1992-06-01
影响因子：
3.6
通讯作者：
ELLAR, DJ
中科院分区：
文献类型：
--
作者：
CRICKMORE, N;ELLAR, DJ
苏云金芽孢杆菌cryllA δ-内毒素基因被发现是三基因操纵子中的第三个基因，其中孢子形成依赖性启动子位于第一个基因orf 1的上游。我们在这里表明，中间基因（orf 2）的多肽产物是毒素基因的有效表达所必需的。在不存在功能性ORF 2多肽的情况下，毒素不形成其它已知苏云金芽孢杆菌毒素所特有的结晶内含物。我们讨论了这一发现的重要性方面的可能作用的伴侣蛋白在这些蛋白质的结晶。
The Bacillus thuringiensis cryllA delta-endotoxin gene is found as the third-gene in a three-gene operon, with a sporulation-dependent promoter lying upstream of the first gene, orf1. We show here that the polypeptide product of the middle gene (orf2) is required for efficient expression of the toxin gene. In the absence of a functional ORF2 polypeptide the toxin does not form the crystalline inclusions characteristic of other known Bacillus thuringiensis toxins. We discuss the importance of this finding with respect to the possible role of chaperonins in the crystallization of these proteins.