X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I.

X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I.
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X 射线增感屏极大地增强了放射性同位素 32P 和 125I 放射自显影的检测。

DOI:
10.1016/0003-2697(78)90333-0
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发表时间:
1978
影响因子:
2.9
通讯作者:
P. Shank
P. Shank
中科院分区:
生物学4区
文献类型:
--
作者:
Ronald Swanstrom;P. Shank

文献摘要

被引文献

相似文献

X射线增感屏的使用显著地增强了放射自显影术对γ射线(125 I)和高能β(32 P)粒子发射放射性同位素的探测。我们比较了五种市售的增感屏(含有不同的无机荧光粉),并结合了一些胶片和曝光条件。当在−70°C下用柯达RP皇家X-OMAT胶片拍摄放射自显影照片时,一个钨酸钙(杜邦,Cronex Lighting Plus增感屏)在32 P的检测中产生8至10倍的增强,在125 I的检测中产生30至40倍的增强。用一个增感屏,在20小时的曝光中,在10平方毫米的面积内,10 dpm的32 P和50 dpm的125 I很容易被探测到。
Detection of both γ-ray (125I) and high energy β (32P) particle-emitting radioisotopes by autoradiography is significantly enhanced by the use of X-ray intensifying screens. We have compared five commercially available intensifying screens (containing different inorganic phosphors) in combination with a number of films and exposure conditions. One calcium tungstate (Dupont, Cronex Lighting Plus intensifying screen produces an 8- to 10-fold enhancement in the detection of32P and a 30- to 40-fold enhancement in the detection of125I when an autoradiogram is made with Kodak RP ROYAL X-OMAT film at −70°C. With one intensifying screen, 10 dpm of32P and 50 dpm of125I in an area of 10 mm2are readily detectable in a 20-hr exposure.